Abstract
Analyzing individual cells allows detecting a minor group of abnormal cells present in a large population of normal cells. This ability can be essential to understanding diseases, such as cancer and diabetes. Microchip electrophoresis (MCE) is the technique of choice for single-cell analysis. However, since the channels in microfluidic devices are very small, achieving the desired assay sensitivity on a microfluidic platform remains a challenge. Here, we describe an MCE method with highly sensitive chemiluminescence detection for simultaneous determination of multiple amino acids present in single cells.
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Financial support from National Institutes of Health (SC1 GM089557) is gratefully acknowledged.
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Liu, YM., Zhao, S. (2012). Quantification of Amino Acids in a Single Cell by Microchip Electrophoresis with Chemiluminescence Detection. In: Alterman, M., Hunziker, P. (eds) Amino Acid Analysis. Methods in Molecular Biology, vol 828. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-445-2_28
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DOI: https://doi.org/10.1007/978-1-61779-445-2_28
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Publisher Name: Humana Press, Totowa, NJ
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