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Measurement of Proton Leak and Electron Leak in Isolated Mitochondria

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Mitochondrial Bioenergetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 810))

Abstract

Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton and electron leaks. In this chapter, methods are presented to measure proton and electron leak activities in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasised.

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Acknowledgments

We thank Julie Buckingham for useful advice on the preparation of TPMP sleeves.

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Authors and Affiliations

  1. Buck Institute for Research on Aging, 8001 Redwood Blvd., Novato, CA, 94945-1400, USA

    Charles Affourtit, Casey L. Quinlan & Martin D. Brand

Authors
  1. Charles Affourtit
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  2. Casey L. Quinlan
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  3. Martin D. Brand
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Corresponding author

Correspondence to Martin D. Brand .

Editor information

Editors and Affiliations

  1. Center for Neurosciences and Cell Biolog, Universidad de Coimbra, Lg. Marquês de Pombal 3046, Coimbra, 3004-517, Portugal

    Carlos M. Palmeira

  2. Centro de Neurociencias, Universidad de Coimbra, Lg. Marquês de Pombal 3046, Coimbra, 3004-517, Portugal

    António J. Moreno

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Affourtit, C., Quinlan, C.L., Brand, M.D. (2012). Measurement of Proton Leak and Electron Leak in Isolated Mitochondria. In: Palmeira, C., Moreno, A. (eds) Mitochondrial Bioenergetics. Methods in Molecular Biology, vol 810. Humana Press. https://doi.org/10.1007/978-1-61779-382-0_11

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  • DOI: https://doi.org/10.1007/978-1-61779-382-0_11

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-381-3

  • Online ISBN: 978-1-61779-382-0

  • eBook Packages: Springer Protocols

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