Abstract
Supported or modified enzymes in the form of mobile nanoparticles are designed for enhanced activities and stabilities; however, their practical operations are dwarfed due to their tiny size which always makes recycling an arduous task and a potential risk to the environment. To overcome such drawbacks, this chapter describes a method for the preparation of a new form of microcapsules, possessing single-cavity compartments and nano-pores in the shell, to encage nanoparticle-based biocatalysts and form cell-like microreactors (CLMRs). The encaged nanoscale catalysts are maintained their high activities as in a bulk-phase solution, while they could be handled as materials of sizes hundreds-fold larger.
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Acknowledgments
The authors thank the National Basic Research Program of China (New 973 Program, Contract Nos. 2009CB724705) and Chinese National Science Foundation of China (20576135, 20536050, and 20728607) for their support. The support from Chinese Academy of Sciences for international collaboration is also greatly appreciated.
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Gao, F., Wang, P., Ma, G. (2011). Microencapsulation of Bioactive Nanoparticles. In: Wang, P. (eds) Nanoscale Biocatalysis. Methods in Molecular Biology, vol 743. Humana Press. https://doi.org/10.1007/978-1-61779-132-1_13
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DOI: https://doi.org/10.1007/978-1-61779-132-1_13
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