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Pluripotent Stem Cells from the Postnatal Testis: Unlocking the Potential of Spermatogonial Stem Cells

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Male Germline Stem Cells: Developmental and Regenerative Potential

Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

While embryonic stem (ES) cells are well known to give rise to tissues comprising all three germ layers, only recently was it shown that cells from the postnatal testis could produce embryonic-like stem cells in culture. The latter, arising in vitro from spermatogonia, can undertake most, if not all, the functions of ES cells. This chapter explores the potential predisposing factors for postnatal germ cells to become pluripotent, including expression of pluripotency-associated genes and epigenetic factors. The major published studies describing the production of ES-like cells from mice and human tissues are reviewed. Finally, we assess the data demonstrating functionality of the differentiated derivatives of ES-like cells. The possible uses of testis-derived stem cells for the study of pluripotency and for regenerative applications is also discussed in comparison to other approaches using ES cells and induced pluripotent stem (ips) cells.

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Acknowledgments

This work was supported by the Howard Hughes Medical Institute, the Ansary Center for Stem Cell Therapeutics, and National Heart, Lung and Blood Institute grants HL075234, HL059312, and HL084936 (S.R.), and the ASCO Young Investigator Award (M.S.). Marco Seandel is a Stanley and Fiona Druckenmiller New York Stem Cell Foundation Fellow.

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  1. Department of Surgery, Weill Medical College of Cornell University, 1300 York Avenue, Room LC-703, New York, NY, 10065, USA

    Marco Seandel

  2. Howard Hughes Medical Institute, Ansary Center for Stem Cell Therapeutics, and Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, Room A-863, New York, NY, 10065, USA

    Shahin Rafii

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  1. Marco Seandel
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  2. Ilaria Falciatori
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Correspondence to Marco Seandel or Shahin Rafii .

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  1. Magee-Womens Research Institute, University of Pittsburgh, Craft Avenue 204, Pittsburgh, 15213, Pennsylvania, USA

    Kyle E. Orwig

  2. Magee-Womens Research Institute, University of Pittsburgh, Craft Avenue 204, Pittsburgh, 15213, Pennsylvania, USA

    Brian P. Hermann

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Seandel, M., Falciatori, I., Rafii, S. (2011). Pluripotent Stem Cells from the Postnatal Testis: Unlocking the Potential of Spermatogonial Stem Cells. In: Orwig, K., Hermann, B. (eds) Male Germline Stem Cells: Developmental and Regenerative Potential. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-973-4_2

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