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Adipogenic Differentiation of Human Adipose-Derived Stem Cells on 3D Silk Scaffolds

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Adipose-Derived Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 702))

Abstract

Current treatment modalities for soft tissue defects due to various pathologies and trauma include autologous grafting and the use of commercially available fillers. However, these treatment methods are associated with a number of limitations, such as donor site morbidity and volume loss over time. As such, improved therapeutic options are needed. Tissue engineering techniques offer novel solutions to these problems through development of bioactive tissue constructs that can regenerate adipose tissue with an appropriate structure and function. The recent advances in the derivation and characterization of hASCs have led to numerous studies of soft tissue reconstruction. In this chapter, we discuss methods in which our laboratory has used hASCs and silk scaffolds for adipose tissue engineering. The use of naturally occurring and clinically acceptable materials such as silk protein for tissue-engineering applications poses advantages with respect to biocompatibility and mechanical and biological properties.

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Acknowledgments

We would like to thank the NIH P41 Tissue Engineering Resource Center (P41 EB002520) and AFIRM for support of soft tissue engineering research.

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Authors and Affiliations

  1. Biomedical Engineering, Tufts University, Medford, MA, USA

    Jennifer H. Choi

  2. Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA

    Evangelia Bellas

  3. Biomedical Engineering, Vanderbilt Clinic, Columbia University, New York, NY, USA

    Gordana Vunjak-Novakovic

  4. Bioengineering Department, Tufts University, Medford, MA, USA

    David L. Kaplan

Authors
  1. Jennifer H. Choi
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  2. Evangelia Bellas
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  3. Gordana Vunjak-Novakovic
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  4. David L. Kaplan
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Corresponding author

Correspondence to David L. Kaplan .

Editor information

Editors and Affiliations

  1. , Stem Cell Biology, Pennington Biomedical Research Center, Perkins Rd. 6400, Baton Rouge, 70808, Louisiana, USA

    Jeffrey M. Gimble

  2. School of Medicine, Department of Pharmacology, Tulane University, Tulane Ave. 1430, New Orleans, 70112-2699, Louisiana, USA

    Bruce A. Bunnell

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Choi, J.H., Bellas, E., Vunjak-Novakovic, G., Kaplan, D.L. (2011). Adipogenic Differentiation of Human Adipose-Derived Stem Cells on 3D Silk Scaffolds. In: Gimble, J., Bunnell, B. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 702. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-960-4_23

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  • DOI: https://doi.org/10.1007/978-1-61737-960-4_23

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-959-8

  • Online ISBN: 978-1-61737-960-4

  • eBook Packages: Springer Protocols

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