Abstract
There are several types of bioreactors currently available for the culture of orthopaedic tissue engineered constructs. These vary from the simple to the complex in design and culture. Preparation of samples for bioreactors varies depending on the system being used. This chapter presents data and describes tried and tested methodologies for the preparation of 3D samples for a Rotatory Synthecon Bioreactor (Cellon), a plate shaker, a perfusion system, and a Bose Electroforce Systems Biodynamic Instrument for the in vitro culture of bone and ligament tissue.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Stephens, J. S., Cooper, J. A., Phelan, F. R., Jr., and Dunkers, J. P. (2007) Perfusion flow bioreactor for 3D in situ imaging: investigating cell/biomaterials interactions. Biotechnol. Bioeng. 97, 952–961.
Jones, G. and Cartmell, S. H. (2006) Optimization of cell seeding efficiencies on a three-dimsional gelatin scaffold for bone tissue engineering. J. Appl. Biomater. Biomech. 4, 172–180.
Cartmell, S. H., Porter, B. D., Garcia, A. J., and Guldberg, R. E. (2003) Effects of medium perfusion rate on cell-seeded three-dimensional bone constructs in vitro. Tissue Eng. 9, 1197–1203.
Porter, B., Zauel, R., Stockman, H., Guldberg, R., and Fyhrie, D. (2005) 3-D computational modeling of media flow through scaffolds in a perfusion bioreactor. J. Biomech. 38, 543–549.
Whittaker, R. J., Booth, R., Dyson, R., Bailey, C., Parsons Chini, L., Naire, S., Payvandi, S., Rong, Z., Woollard, H., Cummings, L. J., Waters, S. L., Mawasse, L., Chaudhuri, J. B., Ellis, M. J., Michael, V., Kuiper, N. J., and Cartmell, S. (2009) Mathematical modelling of fibre-enhanced perfusion inside a tissue-engineering bioreactor. J. Theor. Biol. 256, 533–546.
Wendt, D., Stroebel, S., Jakob, M., John, G. T., and Martin, I. (2006) Uniform tissues engineered by seeding and culturing cells in 3D scaffolds under perfusion at defined oxygen tensions. Biorheology 43, 481–488.
Timmins, N. E., Scherberich, A., Fruh, J. A., Heberer, M., Martin, I., and Jakob, M. (2007) Three-dimensional cell culture and tissue engineering in a T-CUP (tissue culture under perfusion). Tissue Eng. 13, 2021–2028.
Alvarez-Barreto, J. F., Linehan, S. M., Shambaugh, R. L., and Sikavitsas, V. I. (2007) Flow perfusion improves seeding of tissue engineering scaffolds with different architectures. Ann. Biomed. Eng. 35, 429–442.
Johnson, I. (2002) Fuorescent staining of living cells, in Microscopy and Histology for Molecular Biologists (Kiernan, J. A., and Mason, I., Eds.), pp. 92–101, Portland Press, London.
Vlierberghe, S. V., Cnudde, V., Dubruel, P., Masschaele, B., Cosijns, A., Paepe, I. D., Jacobs, P. J., Hoorebeke, L. V., Remon, J. P., and Schacht, E. (2007) Porous gelatin hydrogels: 1. Cryogenic formation and structure analysis. Biomacromolecules 8, 331–337.
Acknowledgements
The authors wish to acknowledge a private local charity, the Royal Society, and the BBSRC (BBS/S/M/2006/13131 and BB/F013892/1) for financial assistance. The authors also wish to acknowledge the contribution of Mr. Michael Vipin and Dr. Nikki Kuiper (Keele University, UK) for providing the perfusion bioreactor seeding efficiency data. We wish to acknowledge Swann Morton for assistance in gamma sterilisation of the PCL/glass fibre composites and Dr. Alex Fotheringham at Herriot Watt University for plasma sterilisation. We also wish to acknowledge the contribution of the hydroxyapatite scaffold fabrication by Dr. Irene Turner and Dr. Jon Gittings at the University of Bath. Finally, we wish to acknowledge the financial and technical assistance of Mr. David Healy at Giltech Ltd. for the fabrication of the PCL/glass fibre scaffolds.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Cartmell, S.H., Rathbone, S., Jones, G., Hidalgo-Bastida, L.A. (2011). 3D Sample Preparation for Orthopaedic Tissue Engineering Bioreactors. In: Haycock, J. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 695. Humana Press. https://doi.org/10.1007/978-1-60761-984-0_5
Download citation
DOI: https://doi.org/10.1007/978-1-60761-984-0_5
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-60761-983-3
Online ISBN: 978-1-60761-984-0
eBook Packages: Springer Protocols