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Tandem Mass Spectrometric Methods for Phospholipid Analysis from Brain Tissue

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Mass Spectrometry Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 656))

Abstract

We describe the utility of intermediate-pressure MALDI and tandem mass spectrometry (MS/MS and MSn) for the characterization and imaging of phospholipids in brain tissue sections. The use of both MS/MS spectra and MS/MS images allows for identification of isobaric compounds. The structural characterization of phosphatidylcholines, phosphatidylserines, phosphatidylethanolamines, and sphingomyelins directly from tissue sections is described.

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Acknowledgments

The authors wish to acknowledge the assistance of George Stafford and Mari Prieto-Conaway at ThermoFisher Scientific (San Jose, CA, USA). We thank Dr. Nigel Calcutt of the University of California San Diego for donation of the tissue specimens. The NIH is greatly acknowledged for support (NIH RO1 ES007355).

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Authors and Affiliations

  1. GCRC Core Laboratory, Department of Medicine, University of Florida, Gainesville, FL, USA

    Timothy J. Garrett

  2. Department of Chemistry, University of Florida, Gainesville, FL, USA

    Richard A. Yost

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  1. Timothy J. Garrett
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  2. Richard A. Yost
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Editor information

Editors and Affiliations

  1. , Beckman Institute, Univ. of Illinois at Urbana-Champaign, N. Mathews Avenue 405, Urbana, 61801, Illinois, USA

    Stanislav S. Rubakhin

  2. , Department of Chemistry, Univ. of Illinois at Urbana-Champaign, South Mathews Avenue 63-5 600, Urbana, 61801, Illinois, USA

    Jonathan V. Sweedler

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Garrett, T.J., Yost, R.A. (2010). Tandem Mass Spectrometric Methods for Phospholipid Analysis from Brain Tissue. In: Rubakhin, S., Sweedler, J. (eds) Mass Spectrometry Imaging. Methods in Molecular Biology, vol 656. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-746-4_12

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  • DOI: https://doi.org/10.1007/978-1-60761-746-4_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-745-7

  • Online ISBN: 978-1-60761-746-4

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