Abstract
It is widely accepted that cationic antimicrobial peptides possess potent microbicidal properties. Recent studies show that in addition to their antimicrobial action, these peptides can exhibit anti-inflammatory activity. The purpose of this chapter is to describe in vivo ear inflammation models that can be used for evaluating the anti-inflammatory activity of antimicrobial peptides. The models are based on different mechanisms of inflammation development and include irritant dermatitis (a model induced by a single application of 12-o-tetradecanoylphorbol acetate [TPA]) and allergic dermatitis, or delayed type hypersensitivity reaction (a model induced by repetitive application of oxazolone).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Scott, M. G., Rosenberger, C. M., Gold, M. R., Finlay, B. B., and Hancock, R. E. (2000) An alpha-helical cationic peptide selectively modulates macrophage responses to lipopolysaccharide and directly alters macrophage gene expression. J. Immunol. 165, 3358–3365.
Scott, M. G., Davidson, D. J., Gold, M. R., Bowdish, D., and Hancock, R. E. (2002) The human antimicrobial peptide LL-37 is a multifunctional modulator of innate immune responses. J. Immunol. 169, 3883–3891.
McInturff, J. E., Wang, S. J., Machleidt, T., Lin, T. R., Oren, A., Hertz, C. J., Krutzik, S. R., Hart, S., Zeh, K., Anderson, D. H., Gallo, R. L., Modlin, R. L., and Kim, J. (2005) Granulysin-derived peptides demonstrate antimicrobial and anti-inflammatory effects against Propionibacterium acnes. J. Invest. Dermatol. 125, 256–263.
Masera, R. G., Bateman, A., Muscettola, M., Solomon, S., and Angeli, A. (1996) Corticostatins/defensins inhibit in vitro NK activity and cytokine production by human peripheral blood mononuclear cells. Regul. Pept. 62, 13–21.
Guarna, M. M., Coulson, R., and Rubinchik, E. (2006) Anti-inflammatory activity of cationic peptides: application to the treatment of acne vulgaris. FEMS Microbiol. Lett. 257, 1–6.
Scott, M. G., Lee, K., Maclean, S., Gong, J., Finlay, B. B., and Hancock, R. E. W. (2003) Immunomodulatory peptides that protect in murine models of infection. Abstr 43rd Intersci. Conf. Antimicrob. Agents Chemother. 43, 62.
McNicol, P. J., Rubinchik, E., Pasetka, C., Barger, R., Lopez, J., and Friedland, H. D. (2001) MBI 594AN is active against Acne vulgaris organisms, does not induce resistance and has anti-inflammatory activity in vivo. Abstr 41st Intersci. Conf. Antimicrob. Agents Chemother. 41, 205.
Rao, T. S., Currie, J. L., Shaffer, A. F., and Isakson, P. C. (1993) Comparative evaluation of arachidonic acid (AA)- and tetradecanoylphorbol acetate (TPA)-induced dermal inflammation Inflammation 17, 723–741.
Tramposch, K. M. (1999) Skin inflammation. In In vivo models of inflammation. Morgan, D. W., Marshall, L. A. (Eds.), pp. 179–204. Basel, Switzerland: Birkhauser Verlag.
Carlson R. P., O’Neill-Davis, L., Chang, J., and Lewis, A. J. (1985) Modulation of mouse ear edema by cyclooxygenase and lipoxygenase inhibitors and other pharmacologic agents Agents Actions 17, 197–204.
Krieger, T. J., and McNicol, P. J. (2004) Antimicrobial cationic peptides and formulations thereof. Patent number US 6,835,536 B2.
Young, J. M., Wagner, B. M., and Spires, D. A. (1983) Tachyphylaxis in 12-o-tetradecanoylphorbol acetate- and arachidonic acid-induced ear edema J. Invest. Dermatol. 80, 48–52.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Rubinchik, E., Pasetka, C. (2010). Assay Systems for Measurement of Anti-inflammatory Activity. In: Giuliani, A., Rinaldi, A. (eds) Antimicrobial Peptides. Methods in Molecular Biology, vol 618. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-594-1_21
Download citation
DOI: https://doi.org/10.1007/978-1-60761-594-1_21
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-593-4
Online ISBN: 978-1-60761-594-1
eBook Packages: Springer Protocols