Abstract
Chondroitin/dermatan sulfate (CS/DS) glycosaminoglycans (GAGs) are present in high levels in connective tissue where they play roles as structural molecules and in protein-binding interactions. Recent developments in the techniques for analysis of CS/DS using capillary electrophoresis (CE) have enabled progress in the understanding of changes in CS/DS structure that accompany connective tissue diseases including osteoarthritis. Key to these developments is the ability to extract CS/DS GAGs from small quantities of connective tissue. This chapter describes a method for connective tissue GAG extraction, derivatization, and workup for subsequent capillary electrophoretic and/or mass spectrometric analysis.
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The authors were supported by NIH grant P41RR10888 and NIH contract NO1HV28178.
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Bielik, A.M., Zaia, J. (2010). Extraction of Chondroitin/Dermatan Sulfate Glycosaminoglycans from Connective Tissue for Mass Spectrometric Analysis. In: Li, J. (eds) Functional Glycomics. Methods in Molecular Biology, vol 600. Humana Press. https://doi.org/10.1007/978-1-60761-454-8_15
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