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Measurement of 8-Isoprostane in Exhaled Breath Condensate

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Advanced Protocols in Oxidative Stress II

Part of the book series: Methods in Molecular Biology ((MIMB,volume 594))

Abstract

Oxidative stress is functionally involved in the pathophysiology of lung diseases including asthma and chronic obstructive pulmonary disease. 8-Isoprostane, which is derived from free radical-catalyzed peroxidation of arachidonic acid, is one of the most reliable biomarkers of oxidative stress. Exhaled breath condensate (EBC) is a completely noninvasive method for collecting airway secretions. We developed a specific and sensitive radioimmunoassay (RIA) that has been applied to the measurement of 8-isoprostane in EBC. This RIA for 8-isoprostane has been validated using high performance liquid chromatography. Measurement of 8-isoprostane in EBC is a useful noninvasive technique for exploring the role of oxidative stress in lung diseases. This technique might provide important insights into the understanding of the clinical pharmacology of antioxidants and might be useful for monitoring the effects of pharmacological therapy.

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References

  1. Halliwell B, Grootveld M (1987) The measurement of free radical reactions in humans. FEBS Lett 213:9–14

    Article  PubMed  CAS  Google Scholar 

  2. Montuschi P, Barnes PJ (2006) Isoprostanes and asthma. Drug Discov Today: Ther Strateg 3:287–292

    Article  Google Scholar 

  3. Rahman I, Adcock IM (2006) Oxidative stress and redox regulation of lung inflammation in COPD. Eur Respir J 28:219–242

    Article  PubMed  CAS  Google Scholar 

  4. Cantin AM, White TB, Cross CE, Forman HJ, Sokol RJ, Borowitz D (2007) Antioxidants in cystic fibrosis. Conclusions from the CF antioxidant workshop, Bethesda, Maryland, November 11–12. Free Radic Biol Med 42:15–31

    CAS  Google Scholar 

  5. Morrow JD, Hill KE, Burk RF, Nammour TM, Badr KF, Roberts LJ, II. (1990) A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanism. Proc Natl Acad Sci USA 87:9383–9387

    Google Scholar 

  6. Montuschi P, Barnes PJ, Roberts LJ, II (2004) Isoprostanes: markers and mediators of oxidative stress. FASEB J 18:1791–1800

    Google Scholar 

  7. Montuschi P, Barnes PJ, Roberts LJ, II (2007) Insights into oxidative stress: the isoprostanes. Curr Med Chem 14:703–717

    Google Scholar 

  8. Montuschi P, Corradi M, Ciabattoni G, Nightingale J, Kharitonov SA, Barnes PJ (1999) Increased 8-isoprostane, a marker of oxidative stress, in exhaled condensate of asthma patients. Am J Respir Crit Care Med 160:216–220

    PubMed  CAS  Google Scholar 

  9. Mondino C, Ciabattoni G, Koch P, Pistelli R, Trové A, Barnes PJ, Montuschi P (2004) Effects of inhaled corticosteroids on exhaled leukotrienes and prostanoids in asthmatic children. J Allergy Clin Immunol 114:761–767

    Article  PubMed  CAS  Google Scholar 

  10. Shahid SK, Kharitonov SA, Wilson NM, Bush A, Barnes PJ (2005) Exhaled 8-isoprostane in childhood asthma. Respir Res 6:79

    Article  PubMed  Google Scholar 

  11. Montuschi P, Collins JV, Ciabattoni G, Lazzeri N, Corradi M, Kharitonov SA, Barnes PJ (2000) Exhaled 8-isoprostane as an in vivo biomarker of lung oxidative stress in patients with COPD and healthy smokers. Am J Respir Crit Care Med 162:1175–1177

    PubMed  CAS  Google Scholar 

  12. Montuschi P, Kharitonov SA, Ciabattoni G, Corradi M, van Rensen L, Geddes DM, Hodson ME, Barnes PJ (2000) Exhaled 8-isoprostane as a new non invasive biomarker of oxidative stress in cystic fibrosis. Thorax 55:205–209

    Article  PubMed  CAS  Google Scholar 

  13. Lucidi P, Ciabattoni G, Bella S, Barnes PJ, Montuschi P (2008) Exhaled 8-isoprostane and prostaglandin E2 in patients with stable and unstable cystic fibrosis. Free Radic Biol Med 45:913–919

    Article  PubMed  CAS  Google Scholar 

  14. Montuschi P (2002) Indirect monitoring of lung inflammation. Nat Rev Drug Discov 1:238–242

    Article  PubMed  CAS  Google Scholar 

  15. Montuschi P, Barnes PJ (2002) Analysis of exhaled breath condensate for monitoring airway inflammation. Trends Pharmacol Sci 23:232–237

    Article  PubMed  CAS  Google Scholar 

  16. Montuschi P (ed) (2004) New perspectives in monitoring lung inflammation: analysis of exhaled breath condensate. CRC, Boca Raton, FL

    Google Scholar 

  17. Montuschi P (2007) Analysis of exhaled breath condensate in respiratory medicine: Metho­do­logical aspects and potential clinical applications. Ther Adv Respir Dis 1:5–23

    Article  PubMed  CAS  Google Scholar 

  18. Montuschi P, Ragazzoni E, Valente S, Corbo G, Mondino C, Ciappi G, Ciabattoni G (2003) Validation of 8-isoprostane and prostaglandin E2 measurements in exhaled breath condensate. Inflamm Res 52:502–506

    Article  PubMed  CAS  Google Scholar 

  19. Montuschi P, Barnes PJ (2002) Exhaled leukotrienes and prostaglandins in asthma. J Allergy Clin Immunol 109:615–620

    Article  PubMed  CAS  Google Scholar 

  20. Ciabattoni G (1987) Production of antisera by conventional techniques. In: Patrono C, Peskar BA (eds) Radioimmunoassay in basic and clinical pharmacology, Handbook of experimental pharmacology. Springer, Berlin, pp 23–68

    Chapter  Google Scholar 

  21. Wang Z, Ciabattoni G, Créminon C, Lawson J, Fitzgerald GA, Patrono C, Maclouf J (1995) Immunological characterization of urinary 8-epi-prostaglandin F2α excretion in man. J Pharmacol Exp Ther 275:94–100

    PubMed  CAS  Google Scholar 

  22. Ciabattoni G, Pugliese F, Spaldi M, Cinotti GA, Patrono C (1979) Radioimmunoassay measurement of prostaglandins E2 and F2α in human urine. J Endocrinol Invest 2:173–182

    PubMed  CAS  Google Scholar 

  23. Montuschi P, Currò D, Ragazzoni E, Preziosi P, Ciabattoni G (1999) Anaphylaxis increases 8-epi-prostaglandin F2α release from guinea-pig lung in vitro. Eur J Pharmacol 365:59–64

    Article  PubMed  CAS  Google Scholar 

  24. Pradelles P, Grassi J, Maclouf J (1990) Enzyme immunoassays of eicosanoids using acetylcholinesterase. Methods Enzymol 187:24–34

    Article  PubMed  CAS  Google Scholar 

  25. Vaitukaitis J, Robbins JB, Nieschlag E, Ross GT (1971) A method for producing specific antisera with small doses of immunogen. J Clin Endocrinol Metab 33:988–991

    Article  PubMed  CAS  Google Scholar 

  26. Morrow JD, Scruggs J, Chen Y, Zackert WE, Roberts LJII (1998) Evidence that the E2-isoprostane, 15–E2t-isoprostane (8-iso-prostaglandin E2) is formed in vivo. J Lipid Res 39:1589–1593

    PubMed  CAS  Google Scholar 

  27. Montuschi P, Macagno F, Parente P, Valente S, Lauriola L, Ciappi G, Kharitonov SA, Barnes PJ, Ciabattoni G (2005) Effects of cyclo-oxygenase inhibition on exhaled eicosanoids in patients with COPD. Thorax 60:827–833

    Article  PubMed  CAS  Google Scholar 

  28. Montuschi P (2004) Isoprostanes, prostanoids, and leukotrienes in exhaled breath condensate. In: Montuschi P (ed) New perspectives in monitoring lung inflammation: analysis of exhaled breath condensate. CRC Press, Boca Raton, FL, pp 53–66

    Google Scholar 

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Acknowledgments

This work was funded by Catholic University of the Sacred Heart, “Fondi di Ateneo” 2007–2009.

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Authors and Affiliations

  1. Department of Pharmacology, Catholic University of the Sacred Heart, Rome, Italy

    Paolo Montuschi (Faculty of Medicine)

  2. Department of Thoracic Medicine, Imperial College, School of Medicine at the National Heart and Lung Institute, London, UK

    Peter J. Barnes

  3. Department of Drug Sciences, School of Pharmacy, University “G. D’Annunzio”, Chieti, Italy

    Giovanni Ciabattoni

Authors
  1. Paolo Montuschi
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  2. Peter J. Barnes
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  3. Giovanni Ciabattoni
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Corresponding author

Correspondence to Paolo Montuschi .

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Editors and Affiliations

  1. Golden Leaf Rd. 215, Mars Hill, 28754, USA

    Donald Armstrong

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Montuschi, P., Barnes, P.J., Ciabattoni, G. (2010). Measurement of 8-Isoprostane in Exhaled Breath Condensate. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress II. Methods in Molecular Biology, vol 594. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-411-1_5

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  • DOI: https://doi.org/10.1007/978-1-60761-411-1_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-410-4

  • Online ISBN: 978-1-60761-411-1

  • eBook Packages: Springer Protocols

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