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pO2 and ROS/RNS Measurements in the Microcirculation in Hypoxia

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Advanced Protocols in Oxidative Stress II

Part of the book series: Methods in Molecular Biology ((MIMB,volume 594))

Abstract

We expose methods for in vivo assessment of oxygen, nitric oxide (NO), and reactive oxygen species (ROS)/reactive nitrogen species (RNS), in the microcirculation during normoxia and hypoxia. We provide an example of the related mechanisms of ROS/RNS and oxygen level in the process of regulating capillary perfusion. Namely, we discuss the real time pO2 measurements in vivo in the microvessels and tissues of the hamster cheek pouch and window chamber preparations during normoxia and hypoxia, as well as the corresponding changes in ROS/RNS in systemic blood during normoxia and hypoxia under conditions where NO availability is maximally reduced.

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Authors and Affiliations

  1. CNR Institute of Clinical Physiology, University of Pisa, Pisa, Italy

    Silvia Bertuglia (Faculty of Medicine)

  2. Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA

    Marcos Intaglietta

Authors
  1. Silvia Bertuglia
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  2. Marcos Intaglietta
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Correspondence to Silvia Bertuglia .

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Editors and Affiliations

  1. Golden Leaf Rd. 215, Mars Hill, 28754, USA

    Donald Armstrong

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Bertuglia, S., Intaglietta, M. (2010). pO2 and ROS/RNS Measurements in the Microcirculation in Hypoxia. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress II. Methods in Molecular Biology, vol 594. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-411-1_2

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  • DOI: https://doi.org/10.1007/978-1-60761-411-1_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-410-4

  • Online ISBN: 978-1-60761-411-1

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