Abstract
Although recent molecular biology advances provide a very effective way in determining localized gene expression, the visualization of the expression by in situ hybridization of whole-mount embryos or paraffin-embedded tissue sections continues to be an excellent method to determine overall gene expression. In this chapter, I review protocols designed to determine mRNA expression by in situ hybridization techniques, in particular, focusing on the developing stratified epidermis.
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References
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© 2010 Humana Press, a part of Springer Science+Business Media, LLC
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Morasso, M.I. (2010). Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 585. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-380-0_17
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DOI: https://doi.org/10.1007/978-1-60761-380-0_17
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-379-4
Online ISBN: 978-1-60761-380-0
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