Abstract
In this chapter, we will demonstrate the usage of a suite of computational techniques based on a coarse-grained elastic network model by applying them to a monomeric helicase—the NS3 helicase of hepatitis C virus. These techniques allow us to predict and visualize collective domain motions encoded in the protein structures, probe allosteric couplings between key functional sites (such as ATP-binding site and DNA/RNA-binding site of a helicase), and simulate ATP-binding-induced global conformational changes. These general techniques are not only applicable to NS3 but also to other multi-domain protein structures.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Zheng, W. (2009). Computer Modeling of Helicases Using Elastic Network Model. In: Abdelhaleem, M. (eds) Helicases. Methods in Molecular Biology, vol 587. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-355-8_17
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DOI: https://doi.org/10.1007/978-1-60327-355-8_17
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