Overview
- Contains step-by-step instructions that ensure successful results
- Provides tricks of the trade and notes on troubleshooting and avoiding known pitfalls
Part of the book series: Methods in Molecular Biology (MIMB, volume 425)
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Table of contents (25 protocols)
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Front Matter
Keywords
About this book
The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 2 illuminates specific applications of sample preparation tools and fractionation strategies to study a variety of biological systems.
Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delves into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field.
Editors and Affiliations
Bibliographic Information
Book Title: 2D PAGE: Sample Preparation and Fractionation
Book Subtitle: Volume 2
Editors: Anton Posch
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-210-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-60327-209-4Published: 19 March 2008
Softcover ISBN: 978-1-61737-879-9Published: 09 December 2010
eBook ISBN: 978-1-60327-210-0Published: 18 March 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 340
Number of Illustrations: 80 b/w illustrations, 3 illustrations in colour
Topics: Proteomics, Protein Science, Microbiology, Cell Biology, Biochemistry, general, Microbial Genetics and Genomics