Abstract
Biofilm formation is a common complication of the use of prosthetic devices. In clinical settings, biofilms can be comprised of one or more microbial species. In order to investigate the interaction between different species within a biofilm, a reproducible, reliable model system has to be utilized and an appropriate system for species identification applied. The present chapter describes the artificial throat model, a model system for growing mixed species biofilms on shunt prostheses. The model is used in conjugation with fluorescent in situ hybridization (FISH), which facilitates identification and localization of the resident microorganisms within biofilms.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Potera, C. (1999) Forging a link between biofilms and disease. Science 283, 1837–1839.
Douglas, L. J. (2002) Medical importance of biofilms in Candida infections. Rev. Iberoam. Micol. 19, 139–143.
Douglas, L. J. (2003) Candida biofilms and their role in infection. Trends Microbiol. 11, 30–36.
Costerton, J. W., Lewandowski, Z., Caldwell, D. E., Korber, D. R., and Lappin-Scott, H. M. (1995) Microbial biofilms. Annu. Rev. Microbiol. 49, 711–745.
Hawser, S. P., and Douglas, L. J. (1994) Biofilm formation by Candida species on the surface of catheter materials in vitro. Infect. Immun. 62, 915–921.
Hawser, S. P., and Douglas, L. J. (1995). Resistance of Candida albicansbiofilms to antifungal agents in vitro. Antimicrob. Agents Chemother. 39, 2128–2131.
Chandra, J., Kuhn, D. M., Mukherjee, P. K., Hoyer, L. L., McCormick, T., and Ghannoum, M. A. (2001) Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance. J. Bacteriol. 183, 5385–5394.
Ramage, G., Vande Walle, K., Wickes, B. L., and Lopez-Ribot, J. L. (2001) Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrob. Agents Chemother. 45, 2475–2479.
Ramage, G., Vandewalle, K., Bachmann, S. P., Wickes, B. L., and Lopez-Ribot, J. L. 2002. In vitro pharmacodynamic properties of three antifungal agents against preformed Candida albicans biofilms determined by time-kill studies. Antimicrob. Agents Chemother. 46, 3634–3636.
Bachmann, S. P., Ramage, G., Vandewalle, K., Patterson, T. F., Wickes, B. L., and Lopez-Ribot, J. L. (2003) Antifungal combinations against Candida albicans biofilms in vitro. Antimicrob. Agents Chemother. 47, 3657–3659.
Lewis, R. E., Kontoyiannis, D. P., Darouiche, R. O., Raad, I. I., and Prince, R. A. (2002) Antifungal activity of amphotericin B, fluconazole, and voriconazole in an in vitro model of Candida catheter-related bloodstream infection. Antimicrob. Agents Chemother. 46, 3499–3505.
Garcia-Sanchez, S., Aubert, S., Iraqui, I., Janbon, G., Ghigo, J. M., and d'Enfert, C. (2004) Candida albicans biofilms: a developmental state associated with specific and stable gene expression patterns. Eukaryot. Cell 3, 536–545.
Leunisse, C., van Weissenbruch, R., Busscher, H. J., van der Mei, H. C., and Albers, F. W. (1999) The artificial throat: a new method for standardization of in vitro experiments with tracheo-oesophageal voice prostheses. Acta Otolaryngol. 119, 604–608.
Schuster, M., Lohscheller, J., Kummer, P., Hoppe, U., Eysholdt, U., and Rosanowski, F. (2003) Quality of life in laryngectomees after prosthetic voice restoration. Folia Phoniatr. Logop. 55, 211–219.
Mahieu, H. F., van Saene, H. K., Rosingh, H. J., and Schutte, H. K. 1986. Candida vegetations on silicone voice prostheses. Arch. Otolaryngol. Head Neck Surg. 112, 321–325.
Palmer, M. D., Johnson A. P., and Elliott, T. S. (1993) Microbial colonization of Blom-Singer prostheses in postlaryngectomy patients. Laryngoscope 103, 910–914.
Neu, T. R., Verkerke, G. J., Herrmann, I. F., Schutte, H. K., van der Mei, H. C., and Busscher, H. J. (1994) Microflora on explanted silicone rubber voice prostheses: taxonomy, hydrophobicity and electrophoretic mobility. J. Appl. Bacteriol. 76, 521–528.
Natarajan, B., Richardson, M. D., Irvine, B. W., and Thomas, M. (1994) The Provox voice prosthesis and Candida albicans growth: a preliminary report of clinical, mycological and scanning electron microscopic assessment. J. Laryngol. Otol. 108, 666–668.
Van den Hoogen, F. J., Oudes, M. J., Hombergen, G., Nijdam, H. F., and Manni, J. J. (1996). The Groningen, Nijdam and Provox voice prostheses: a prospective clinical comparison based on 845 replacements. Acta Otolaryngol. 116, 119–124.
Ackerstaff, A. H., Hilgers, F. J., Meeuwis, C. A., van der Velden, L. A., van den Hoogen, F. J., Marres, H. A., Vreeburg, G. C., and Manni, J. J. (1999) Multi-institutional assessment of the Provox 2 voice prosthesis. Arch. Otolaryngol. Head Neck Surg. 125, 167–173.
Amann, R., Fuchs, B. M., and Behrens, S. (2001) The identification of microorganisms by fluorescence in situ hybridisation. Curr. Opin. Biotechnol. 12, 231–236.
Aoi, Y. (2002) In situ identification of microorganisms in biofilm communities. J. Biosci. Bioeng. 94, 552–556.
Thurnheer, T., Gmur, R., and Guggenheim, B. (2004) Multiplex FISH analysis of a six-species bacterial biofilm. J. Microbiol. Methods 56, 37–47.
Buijssen, K. J., Harmsen, H. J., van der Mei, H. C., Busscher, H. J., and van der Laan, B. F. (2007) Lactobacilli: Important in biofilm formation on voice prostheses. Otolaryngol. Head Neck Surg. 137, 505–507.
Oosterhof, J. J., Buijssen, K. J., Busscher, H. J., van der Laan, B. F., and van der Mei, H. C. (2006) Effects of quaternary ammonium silane coatings on mixed fungal and bacterial biofilms on tracheoesophageal shunt prostheses. Appl. Environ. Microbiol. 72, 3673–3677.
Van der Mei, H. C., Free, R. H., Elving, G. J., van Weissenbruch, R., Albers, F. W., and Busscher, H. J. (2000) Effect of probiotic bacteria on prevalence of yeasts in oropharyngeal biofilms on silicone rubber voice prostheses in vitro. J. Med. Microbiol. 49, 713–718.
Elving, G. J., van der Mei, H. C., Busscher, H. J., van Weissenbruch, R., and Albers, F. (2003) Influence of different combinations of bacteria and yeasts in voice prosthesis biofilms on air flow resistance. Antonie Van Leeuwenhoek 83, 45–55.
Amann, R. I., Binder, B. J., Olson, R. J., Chisholm, S. W., Devereux, R., and Stahl D. A. (1990) Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56, 1919–1925.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Krom, B.P., Buijssen, K., Busscher, H.J., van der Mei, H.C. (2009). Candida Biofilm Analysis in the Artificial Throat Using FISH. In: Cihlar, R.L., Calderone, R.A. (eds) Candida albicans. Methods in Molecular Biology, vol 499. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-151-6_6
Download citation
DOI: https://doi.org/10.1007/978-1-60327-151-6_6
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-58829-760-0
Online ISBN: 978-1-60327-151-6
eBook Packages: Springer Protocols