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Affinity Precipitation of Proteins Using Metal Chelates

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Affinity Chromatography

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 421))

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Summary

Metal affinity precipitation has been successfully developed as a simple purification process for the proteins that have affinity for the metal ions. The copolymers of vinylimidazole with N-isopropylacrylamide are easily synthesized by radical polymerization. When loaded with Cu(II) and Ni(II) ions, these copolymers are capable of selectively precipitating proteins with natural metal-binding groups or histidine-tagged recombinant proteins.

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Author information

Authors and Affiliations

  1. Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur (IITK), Kanpur, India

    Ashok Kumar

  2. Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, Lund, Sweden

    Igor Yu. Galaev

  3. Centre for Chemistry and Chemical Engineering, Lund University, Lund, Sweden

    Bo Mattiasson

Authors
  1. Ashok Kumar
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  2. Igor Yu. Galaev
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  3. Bo Mattiasson
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Editor information

Editors and Affiliations

  1. BioMarin Pharmaceutical Inc., CA

    Michael Zachariou (Director Project Management) (Director Project Management)

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Kumar, A., Galaev, I.Y., Mattiasson, B. (2008). Affinity Precipitation of Proteins Using Metal Chelates. In: Zachariou, M. (eds) Affinity Chromatography. Methods in Molecular Biology™, vol 421. Humana Press. https://doi.org/10.1007/978-1-59745-582-4_3

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  • DOI: https://doi.org/10.1007/978-1-59745-582-4_3

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-659-7

  • Online ISBN: 978-1-59745-582-4

  • eBook Packages: Springer Protocols

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