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Patch-Clamp Methods and Protocols

  • Book
  • © 2007

Overview

Editors:
  1. Peter Molnar
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    You can also search for this editor in PubMed   Google Scholar

  2. James J. Hickman
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  • Details how electrophysiology can be best utilized in drug discovery and development
  • Introduces applications of the dynamic clamp method
  • Includes an extensive theoretical treatise concerning single channel kinetic analysis

Part of the book series: Methods in Molecular Biology (MIMB, volume 403)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. Pharmacology

    1. Front Matter

      Pages 1-1
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    2. Pharmacological Analysis of Recombinant NR1a/2A and NR1a/2B NMDA Receptors Using the Whole-Cell Patch-Clamp Method

      • László Fodor, József Nagy
      Pages 3-13

    3. Memantine as an Example of a Fast, Voltage-Dependent, Open Channel N-Methyl-d-Aspartate Receptor Blocker

      • Chris G. Parsons, Kate Gilling
      Pages 15-36

    4. Methods for Evaluation of Positive Allosteric Modulators of Glutamate AMPA Receptors

      • Edward R. Siuda, Jennifer C. Quirk, Eric S. Nisenbaum
      Pages 37-57

    5. Automated Voltage-Clamp Technique

      • Andrea Ghetti, António Guia, Jia Xu
      Pages 59-69

    6. Flip-the-Tip

      • Michael Fejtl, Uwe Czubayko, Alexander Hümmer, Tobias Krauter, Albrecht Lepple-Wienhues
      Pages 71-85

    7. The Roboocyte

      • Christine Leisgen, Mike Kuester, Christoph Methfessel
      Pages 87-109

  3. Physiology

    1. Front Matter

      Pages 111-111
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    2. Infrared-Guided Laser Stimulation as a Tool for Elucidating the Synaptic Site of Expression of Long-Term Synaptic Plasticity

      • Gerhard Rammes, Matthias Eder, Walter Zieglgänsberger, Hans-Ulrich Dodt
      Pages 113-122

    3. Single-Cell RT–PCR, a Technique to Decipher the Electrical, Anatomical, and Genetic Determinants of Neuronal Diversity

      • Maria Toledo-Rodriguez, Henry Markram
      Pages 123-139

    4. Mechanosensitive Ion Channels Investigated Simultaneously by Scanning Probe Microscopy and Patch Clamp

      • Matthias G. Langer
      Pages 141-164

    5. Synaptic Connectivity in Engineered Neuronal Networks

      • Peter Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das, James J. Hickman
      Pages 165-173

    6. Modeling of Action Potential Generation in NG108-15 Cells

      • Peter Molnar, James J. Hickman
      Pages 175-184

    7. Whole-Cell Voltage Clamp on Skeletal Muscle Fibers With the Silicone-Clamp Technique

      • Sandrine Pouvreau, Claude Collet, Bruno Allard, Vincent Jacquemond
      Pages 185-194

    8. Determination of Channel Properties at the Unitary Level in Adult Mammalian Isolated Cardiomyocytes

      • Romain Guinamard
      Pages 195-209

    9. Electrophysiological Properties of Embryonic Stem Cells During Differentiation Into Cardiomyocyte-Like Cell Types

      • Antoni C. G. van Ginneken, Arnoud C. Fijnvandraat
      Pages 211-217

    10. Hybrid Neuronal Network Studies Under Dynamic Clamp

      • Alan D. Dorval II, Jonathan Bettencourt, Theoden I. Netoff, John A. White
      Pages 219-231

    11. Cardiac Channelopathies Studied With the Dynamic Action Potential-Clamp Technique

      • Géza Berecki, Jan G. Zegers, Ronald Wilders, Antoni C. G. Van Ginneken
      Pages 233-250

  4. Biophysics

    1. Front Matter

      Pages 251-251
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Keywords

About this book

Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training, and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is organized by the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. Individual techniques are explored within the area that they are applied most often. Researchers will find this text to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.

Reviews

From the reviews:

"This book will be helpful to researchers already working in the field of electrophysiology, but also those coming to this field from pharmacological, physiological, and biophysical areas." - Doody's Book Review Service

"'Patch-clamp: Methods and Protocols’ introduces some common patch-clamp applications. It is intended to assist scientists in identifying problems, which could be best addressed by this technique. The book provides the step-by-step procedures to perform the experiments that answer those questions. … It will be a useful companion for moderately advanced users wishing to extend their skills." (Maria Drigelova, General Physiology and Biophysics, Vol. 27, April, 2008)

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