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Electron Capture Dissociation LC/MS/MS for Bottom-Up Proteomics

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Mass Spectrometry of Proteins and Peptides

Part of the book series: Methods In Molecular Biology ((MIMB,volume 492))

Summary

ECD is a fragmentation technique that exhibits unusual properties. It is useful in bottom–up proteomics as a complement to collisional dissociation for deriving additional sequence information, locating post-translational modifications, and revealing racemization of individual amino acids in a peptide sequence.

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References

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Acknowledgments

The author is grateful to Michael L. Nielsen and Mikhail M. Savitski for help in implementing ECD in proteomics. This work was supported by the Knut and Alice Wallenberg Foundation and Wallenberg Consortium North (WCN2003-UU/SLU-009 and instrumental grant to RZ and Carol Nilsson) as well as the Swedish research council (621-2004-4897, 621-2002-5025, and 621-2003-4877).

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Authors and Affiliations

  1. Laboratory for Biological and Medical Mass Spectrometry, Uppsala Biomedical Centrum, Uppsala, Sweden

    Roman A. Zubarev

Authors
  1. Roman A. Zubarev
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Editor information

Editors and Affiliations

  1. Fundamental & Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA

    Mary S. Lipton

  2. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA

    Ljiljana Paša-Tolic

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Zubarev, R.A. (2009). Electron Capture Dissociation LC/MS/MS for Bottom-Up Proteomics. In: Lipton, M.S., Paša-Tolic, L. (eds) Mass Spectrometry of Proteins and Peptides. Methods In Molecular Biology, vol 492. Humana Press. https://doi.org/10.1007/978-1-59745-493-3_25

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  • DOI: https://doi.org/10.1007/978-1-59745-493-3_25

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-48-0

  • Online ISBN: 978-1-59745-493-3

  • eBook Packages: Springer Protocols

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