Plant Hormones

1. Front Matter

   Pages i-viii

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2. Using Reverse Genetics to Develop Small Knockout Collections for Specific Biological Questions

   • Julian Northey, Peter McCourt

   Pages 1-10

3. Visualizing Auxin Transport Routes in Arabidopsis Leaf Primordia

   • Danielle Marcos, Thomas Berleth

   Pages 11-20

4. Gene Expression Analyses for Elucidating Mechanisms of Hormonal Action in Plants

   • George W. Bassel, Nicholas J. Provart

   Pages 21-37

5. Measurement of Plant Hormones by Liquid Chromatography–Mass Spectrometry

   • Stacey J. Owen, Suzanne R. Abrams

   Pages 39-51

6. Measurement of Abscisic Acid and Gibberellins by Gas Chromatography/Mass Spectrometry

   • Masanori Okamoto, Atsushi Hanada, Yuji Kamiya, Shinjiro Yamaguchi, Eiji Nambara

   Pages 53-60

7. Pull-Down Assays for Plant Hormone Research

   • Stefan Kepinski

   Pages 61-80

8. Binding Assays for Brassinosteroid Receptors

   • Ana Caño-Delgado, Zhi-Yong Wang

   Pages 81-88

9. Binding Assays for Abscisic Acid Receptors

   • Fawzi A. Razem, Robert D. Hill

   Pages 89-99

10. Cytokinin Sensing Systems Using Microorganisms

    • Masayuki Higuchi, Tatsuo Kakimoto, Takeshi Mizuno

    Pages 101-109

11. Hormone-Binding Assay Using Living Bacteria Expressing Eukaryotic Receptors

    • Georgy A. Romanov, Sergey N. Lomin

    Pages 111-120

12. Discovery of Plant MicroRNAs and Short-Interfering RNAs by Deep Parallel Sequencing

    • Padmanabhan Chellappan, Hailing Jin

    Pages 121-132

13. The Use of Chemical Genomics to Investigate Pathways Intersecting Auxin-Dependent Responses and Endomembrane Trafficking in Arabidopsis Thaliana

    • Lorena Norambuena, Glenn R. Hicks, Natasha V. Raikhel

    Pages 133-143

14. Back Matter

    Pages 145-146

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The last 10 years have witnessed an explosion in our understanding of plant h- mones. The often vague models of hormone action developedover decadeshave been replaced in short order by detailed molecular models that include receptors and in many cases downstream signal transduction components. Given the rapid progress in understanding the mechanism of action of plant growth regulators, a technical review of hormone methodology is timely. Our book focuses on genetic, biochemical, ana- tical and chemical biological approaches for understanding and dissecting plant h- mone action. The greatest strides in plant hormone biology have come, by and large, from the use of genetic methods to identify receptors and we dedicate a chapter to general genetic methods of analysis using the model system Arabidopsis thaliana. A cluster of chapters focuses on biochemical methods for documenting interactions betweenhormonesand their receptors. Theimportance of these assays is tremendous; receptor–ligand interactions in animal model systems have been the cornerstones of pharmacological and medicinal chemical assays that have enabled identification of selective and non-selective agonists and antagonists that can be used to further probe and dissect questions of receptor function. This is likely to be a major new frontier in plant hormone research.

• 454 sequencing technology
• Arabidopsis thaliana
• Chemical genetics
• Expression
• Liana
• MicroRNA analysis
• Plant growth regulators
• Signal transduction
• Transport
• gene expression

From the reviews of the second edition: “This book introduces several approaches essential for plant hormone biology at the forefront of research. … This book has been published at an apt time for plant hormone biologists, especially for PhD students and advanced researchers in this field, to move the field to a higher stage and to lead them towards wider and more advanced technical approaches to hormone analyses, to encourage them to accumulate more specific genomic information and to apply more sophisticated analytical tools.” (Tomokazu Koshiba, Annals of Botany, Vol. 105 (4), April, 2010)

• Dept. Botany, University of Toronto, Toronto, Canada

  Sean Cutler, Dario Bonetta

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