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Capillary Electrophoresis With Laser-Induced Fluorescence

Environmental Applications

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Capillary Electrophoresis

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 384))

Summary

Capillary electrophoresis (CE), especially free-zone CE, offers a relatively simple separation with moderate selectivity based on the mobility of ions in solution. Laser-induced fluorescence (LIF) detection, an extremely sensitive technique, can be coupled with a variety of separation conditions to achieve sensitive and quantitative results. When these techniques are combined, CE/LIF provides the sensitivity and increased selectivity that makes trace level environmental analysis of fluorescent compounds possible at or below levels typical for gas chromatography (GC)/mass spectrometry (MS). We offer a panoramic review of the role of these tools in solving environmental and related analytical problems before providing a detailed experimental protocol.

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Acknowledgment

The US Environmental Protection Agency (EPA), through its Office of Research and Development (ORD), funded the work involved in preparing this article. It has been subject to the Agency's peer review and has been approved for publication. The US Government has the right to retain a nonexclusive, royalty-free license in and to any copyright covering this article.

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Authors and Affiliations

  1. National Exposure Research Laboratory, Environmental Sciences Division, US Environmental Protection Agency, Office of Research and Development, Las Vegas, NV

    Lee Riddick & William C. Brumley

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  1. Lee Riddick
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  2. William C. Brumley
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Editor information

Editors and Affiliations

  1. HelmholtzZentrum MĂĽnchen German Research Center for Environmental Health, Neuherberg, Germany

    Philippe Schmitt-Kopplin

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Riddick, L., Brumley, W.C. (2008). Capillary Electrophoresis With Laser-Induced Fluorescence. In: Schmitt-Kopplin, P. (eds) Capillary Electrophoresis. Methods In Molecular Biology™, vol 384. Humana Press. https://doi.org/10.1007/978-1-59745-376-9_6

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  • DOI: https://doi.org/10.1007/978-1-59745-376-9_6

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-539-2

  • Online ISBN: 978-1-59745-376-9

  • eBook Packages: Springer Protocols

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