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High-Throughput Screening of Protein Interaction Networks in the TGFβ Interactome: Understanding the Signaling Mechanisms Driving Tumor Progression

  • Chapter
Transforming Growth Factor-β in Cancer Therapy, Volume II

Part of the book series: Cancer Drug Discovery and Development ((CDD&D))

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Abstract

High-throughput (HT) proteomic techniques allow the study of hundreds to thousands of proteins simultaneously. Several HT methodologies have been developed to determine protein-protein interactions (PPIs) and therefore protein function in mammalian cells. A few of these, including protein complementation assays, mass spectrometry, yeast two-hybrid and luminescence-based mammalian interactome (LUMIER) mapping, have been applied to the study of TGFβ signaling. PPIs revealed with these techniques have been crucial in elucidating novel components of the TGFβ signaling network involved in tissue homeostasis and cancer. A good example of this is the recently described TGFβ/Par6 polarity pathway, which was initially discovered in a LUMIER screen for PPIs. A role of this pathway in the process of epithelial-mesenchymal transition has been demonstrated, suggesting its potential involvement in cancer metastasis. Thus, proteomic data are becoming an essential tool for unraveling the dynamic networks that drive cancer onset and tumor progression.

These authors contributed equally to this work.

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Authors and Affiliations

  1. Program in Molecular Biology and Cancer, Centre for Systems Biology, Samuel Lumenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

    Miriam Barrios-Rodiles PhD & Alicia Viloria-Petit PhD

  2. Division of Signaling Biology, Ontario Cancer Institute, Toronto, Ontario, Canada

    Kevin R. Brown MSc, Igor Jurisica PhD & Jeffrey L. Wrana PhD

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  1. Miriam Barrios-Rodiles PhD
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  2. Alicia Viloria-Petit PhD
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  3. Kevin R. Brown MSc
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  4. Igor Jurisica PhD
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  5. Jeffrey L. Wrana PhD
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Editors and Affiliations

  1. Cancer Training Branch, Office of Centers, Training and Resources, National Cancer Institute, Bethesda, MD

    Sonia B. Jakowlew PhD

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© 2008 Humana Press Inc., Totowa, NJ

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Barrios-Rodiles, M., Viloria-Petit, A., Brown, K.R., Jurisica, I., Wrana, J.L. (2008). High-Throughput Screening of Protein Interaction Networks in the TGFβ Interactome: Understanding the Signaling Mechanisms Driving Tumor Progression. In: Jakowlew, S.B. (eds) Transforming Growth Factor-β in Cancer Therapy, Volume II. Cancer Drug Discovery and Development. Humana Press. https://doi.org/10.1007/978-1-59745-293-9_18

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  • DOI: https://doi.org/10.1007/978-1-59745-293-9_18

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-715-0

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