Abstract
There is massive gene replication predicted for the activation of fatty acids and their entry into the β-oxidation cycle for fatty acid oxidation. These two steps in fatty acid metabolism are catalyzed by FadD and FadE enzymes with 36 genes predicted for each of these respective activities in Mycobacterium tuberculosis. Here we present methods for the cell-free assay of types of enzymes in live bacteria, as well as for fatty acid oxidation overall.
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Acknowledgment
P.R.W. was supported through DEFRA funding.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Wheeler, P.R. (2009). Analyzing Lipid Metabolism: Activation and β-Oxidation of Fatty Acids. In: Parish, T., Brown, A. (eds) Mycobacteria Protocols. Methods in Molecular Biology, vol 465. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-207-6_4
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DOI: https://doi.org/10.1007/978-1-59745-207-6_4
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