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Isolation of Mycobacterium Species Genomic DNA

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Mycobacteria Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 465))

Abstract

A myriad of methods has been reported for the isolation of genomic DNA from Mycobacterium spp.; some methods use mechanical disruption of the bacterial cells, whereas others use some form of chemical or enzymatic lysis. Regardless of the approach, the end points remain efficient breaking of the complex mycobacterial cell wall and release of high-quality DNA that is suitable for manipulation and analyses by molecular genetic techniques. This chapter providers detailed methods for the large and small isolation of mycobacterial genomic DNA.

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Author information

Authors and Affiliations

  1. Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, 80523, USA

    John T. Belisle

  2. Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA

    Spencer B. Mahaffey BSc & Preston J. Hill BSc

Authors
  1. John T. Belisle
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  2. Spencer B. Mahaffey BSc
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  3. Preston J. Hill BSc
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Corresponding author

Correspondence to John T. Belisle .

Editor information

Editors and Affiliations

  1. Queen Mary's School of Med. & Dentistry, Inst. Cell & Molecular Science, Barts and the London, Newark Street 4, London, E1 2AT, United Kingdom

    Tanya Parish

  2. Queen Mary's School of Med. & Dentistry, Inst. Cell & Molecular Science, Barts and the London, Newark Street 4, London, E1 2AT, United Kingdom

    Amanda Claire Brown

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Belisle, J.T., Mahaffey, S.B., Hill, P.J. (2009). Isolation of Mycobacterium Species Genomic DNA. In: Parish, T., Brown, A. (eds) Mycobacteria Protocols. Methods in Molecular Biology, vol 465. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-207-6_1

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  • DOI: https://doi.org/10.1007/978-1-59745-207-6_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-889-8

  • Online ISBN: 978-1-59745-207-6

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