Abstract
The GC or GC-MS method discussed in Chapter 130 can distinguish substituted monosaccharides, but to characterize the position of acyl groups together with the linkages between the monosaccharides, a strategy has been developed to “capture” the substitution pattern by methylation of all free hydroxyl groups. The constituent monosaccharides are then analyzed after hydrolysis, reduction, and acetylation as partially methylated alditol acetates in a procedure known as methylation analysis (1–3).
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Hounsell, E.F., Davies, M.J., Smith, K.D. (2009). Determination of Monosaccharide Linkage and Substitution Patterns by GC-MS Methylation Analysis. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-198-7_131
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DOI: https://doi.org/10.1007/978-1-59745-198-7_131
Publisher Name: Humana Press, Totowa, NJ
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