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Adult Neural Stem Cells

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Neural Stem Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 438))

Summary

Neural stem cells (NSCs) have been identified in the mature central nervous system (CNS), and they reside in specific areas. Cultures of NSCs can be successfully established in vitro by exploiting the NeuroSphere assay. This methodology relies on the continuous exposure of neural cells to mitogens such as epidermal growth factor and fibroblast growth factor-2. Under these conditions, only NSCs and highly undifferentiated progenitors proliferate, whereas committed precursors and terminally differentiated cells are eliminated from the culture. The proper application of this method to the cells allows the establishment of long-term expanding stable NSC lines, starting from different neural tissues as the adult rodent CNS and human brain tumor specimens.

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Author information

Authors and Affiliations

  1. Stem Cell Research Institute (HSR-SCRI), DIBI-H.S., Raffaele, Milan, Italy

    Rossella Galli PhD & Angelo L. Vescovi PhD

  2. Telethon Institute for Gene Therapy (TIGET), DIBIT-San Raffaele Scientific Institute, Milan, Italy

    Angela Gritti PhD

Authors
  1. Rossella Galli PhD
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  2. Angela Gritti PhD
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  3. Angelo L. Vescovi PhD
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Editor information

Editors and Affiliations

  1. Keck School of Medicine, University of Southern California, 2025 Zonal Ave., Los Angeles, CA 90033, USA

    Leslie P. Weiner

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Galli, R., Gritti, A., Vescovi, A.L. (2008). Adult Neural Stem Cells. In: Weiner, L.P. (eds) Neural Stem Cells. Methods in Molecular Biology™, vol 438. Humana Press. https://doi.org/10.1007/978-1-59745-133-8_7

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  • DOI: https://doi.org/10.1007/978-1-59745-133-8_7

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-846-1

  • Online ISBN: 978-1-59745-133-8

  • eBook Packages: Springer Protocols

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