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Forward and Reverse Genetic Analysis of Chlamydia

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Chlamydia trachomatis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2042))

Abstract

Chlamydia is a major etiological agent of human disease that affects millions of individuals worldwide. Historically, our understanding of the mechanisms that contribute to its pathogenesis has been limited. However, the recent development of powerful genetic tools for manipulating Chlamydia has resulted in significant gains in our ability to dissect its virulence mechanisms. These tools have overcome several barriers for manipulating intracellular pathogens and are amenable for the routine genetic engineering of Chlamydia. Here, we provide several detailed protocols for performing genetic analysis in Chlamydia trachomatis allowing investigators to elucidate how this obligate intracellular pathogen causes disease.

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Acknowledgments

This work was supported by NIH grant 1R21AI140019-01 to R.J.B. We thank Raphael H. Valdivia and members of the Valdivia laboratory for their contributions to the development of this work. We also thank Lee Dolat, Victor J. Ocasio, and Samantha E. Bowen for critical feedback.

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Authors and Affiliations

  1. Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA

    Mateusz Kędzior & Robert J. Bastidas

Authors
  1. Mateusz Kędzior
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  2. Robert J. Bastidas
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Corresponding author

Correspondence to Robert J. Bastidas .

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Editors and Affiliations

  1. Department of Microbiology and Immunology, Vet School, Cornell University, Ithaca, NY, USA

    Amanda Claire Brown

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Kędzior, M., Bastidas, R.J. (2019). Forward and Reverse Genetic Analysis of Chlamydia. In: Brown, A. (eds) Chlamydia trachomatis. Methods in Molecular Biology, vol 2042. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9694-0_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9694-0_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9693-3

  • Online ISBN: 978-1-4939-9694-0

  • eBook Packages: Springer Protocols

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