Abstract
Retinoid and rexinoid receptors are known to regulate key processes during development, differentiation, and cell death in vertebrates. However, their contributions to progression of malignant disease remain largely elusive although it is realized that transformed cancer cells, which essentially evade apoptosis, may display altered molecular expressions or functions associated with retinoid signaling. Here, using a progression model of ovarian cancer, we describe a proteomics-based approach including experimental procedures toward identification and validation of altered protein profiles during transformation. Effectively, this specifies loss of RXR-γ during progression of epithelial ovarian cancer.
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Acknowledgments
We thank Dr. R. Srikanth and Ms. Snigdha (Proteomics facility, NCCS) for providing support for MS analyses. Technical assistance by group members Avinash Mali, Mihir Metkar and Manish Kumar is also gratefully acknowledged.
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Kalra, R.S., Bapat, S.A. (2019). Proteomics to Predict Loss of RXR-γ During Progression of Epithelial Ovarian Cancer. In: Ray, S. (eds) Retinoid and Rexinoid Signaling . Methods in Molecular Biology, vol 2019. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9585-1_1
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DOI: https://doi.org/10.1007/978-1-4939-9585-1_1
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