Abstract
Stem compression reduces or terminates the phloem-mediated transport of carbohydrates and other solutes in tree stems, without causing permanent damage to phloem functioning (Henriksson et al. Tree Physiol. 35:1075–1085, 2015). This has been tested on two species of pine trees, with diameters ranging from 3 to 26 cm in a forest in northern Sweden (Henriksson et al. Tree Physiol. 35:1075–1085, 2015) and in Harvard Forest, USA. Halting the phloem transport of trees in a forest is useful for studying tree physiological processes related to, or dependent on, phloem-transported compounds as well as downstream processes, in particular interactions with soil microbes. Phloem compression can be deployed in the lab and field on single trees, subsets, or over larger areas, depending on what is relevant for a particular research question.
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References
Noel ARA (1970) The girdled tree. Bot Rev 36:162–195
Aubrey DP, Teskey RO (2018) Stored root carbohydrates can maintain root respiration for extended periods. New Phytol 218:142–152
Van de Wal BAE, Windt CW, Leroux O, Steppe K (2017) Heat girdling does not affect xylem integrity: an in vivo magnetic resonance imaging study in the tomato peduncle. New Phytol 215:558–568
Swanson CA, Geiger DR (1967) Time course of low temperature inhibition of sucrose translocation in sugar beets. Plant Physiol 42:751–756
Johnsen K, Maier C, Sanchez F, Anderson P, Butnor J, Waring R, Linder S (2007) Physiological girdling of pine trees via phloem chilling: proof of concept. Plant Cell Environ 30:128–134
De Schepper V, Vanhaecke L, Steppe K (2011) Localized stem chilling alters carbon processes in the adjacent stem and in source leaves. Tree Physiol 31:1194–1203
Henriksson N, Tarvainen L, Lim H, Tor-Ngern P, Palmroth S, Oren R, Marshall J, Näsholm T (2015) Stem compression reversibly reduces phloem transport in Pinus sylvestris trees. Tree Physiol 35:1075–1085
Devaux M, Ghashghaie J, Bert D, Lambrot C, Gessler A, Bathellier C, Ogee J, Loustau D (2009) Carbon stable isotope ratio of phloem sugars in mature pine trees throughout the growing season: comparison of two extraction methods. Rapid Commun Mass Spectrom 23:2511–2518
Högberg P, Högberg MN, Göttlicher SG, Betson NR, Keel SG, Metcalfe DB, Campbell C, Schindlbacher A, Hurry V, Lundmark T, Linder S, Näsholm T (2007) High temporal resolution tracing of photosynthate carbon from the tree canopy to forest soil microorganisms. New Phytol 177:220–228
Nikinmaa E, Sievanen R, Höltta T (2014) Dynamics of leaf gas exchange, xylem and phloem transport, water potential and carbohydrate concentration in a realistic 3-D model tree crown. Ann Bot 114:653–666
Sovonick-Dunford S, Lee DR, Zimmermann MH (1981) Direct and indirect measurements of phloem turgor pressure in white ash. Plant Physiol 68:121–126
Wright JP, Fisher DB (1980) Direct measurement of sieve tube turgor pressure using severed aphid stylets. Plant Physiol 65:1133–1135
Xu Q, Chen S, Yunjuan R, Chen S, Liesche J (2018) Regulation of sucrose transporters and phloem loading in response to environmental cues. Plant Physiol 176:930–945
Liu DD, Chao WM, Turgeon R (2012) Transport of sucrose, not hexose, in the phloem. J Exp Bot 63:4315–4320
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Henriksson, N., Rademacher, T.T. (2019). Stem Compression: A Means to Reversibly Reduce Phloem Transport in Tree Stems. In: Liesche, J. (eds) Phloem. Methods in Molecular Biology, vol 2014. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9562-2_24
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DOI: https://doi.org/10.1007/978-1-4939-9562-2_24
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