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Neisseria meningitidis pp 123–133Cite as

The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp.

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1969))

Abstract

Neisseria meningitidis and Neisseria gonorrhoeae are obligate pathogens of the human host. Due to their adaptation to the human host, many factors required for infection are specialized for the human host to the point that natural infection processes are difficult to replicate in animal models. Immortalized human cell lines have been used to identify the host factors necessary for successful colonization of human mucosal surfaces. One such model is the Detroit 562 pharyngeal immortalized cell monolayer model which is used to measure the rate of attachment to and invasion of N. meningitidis and N. gonorrhoeae into epithelial cells. The methodology of this assay, as well as the maintenance of Detroit 562 cells necessary for the experiment, will be described.

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Acknowledgments

This work is supported by the Australian Defence Material Transfer Center (Project Agreement 10.44) and North Atlantic Treaty Organization (SfP-984235) awarded to MST. EK is supported by a Research Training Program (RTP) PhD scholarship from Murdoch University. CK is supported by the Amanda Young Foundation, a not-for-profit organisation, for improved community awareness of meningococcal disease.

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Authors and Affiliations

  1. Marshall Centre for Infectious Diseases Research and Training, School of Biomedical Sciences, University of Western Australia, Perth, WA, Australia

    Emily A. Kibble, Mitali Sarkar-Tyson & Charlene M. Kahler

  2. School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia

    Emily A. Kibble & Geoffrey W. Coombs

Authors
  1. Emily A. Kibble
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  2. Mitali Sarkar-Tyson
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  3. Geoffrey W. Coombs
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  4. Charlene M. Kahler
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Corresponding author

Correspondence to Charlene M. Kahler .

Editor information

Editors and Affiliations

  1. Institute For Glycomics, Griffith University, Southport, QLD, Australia

    Kate L. Seib

  2. Institute For Glycomics, Griffith University, Southport, QLD, Australia

    Ian R. Peak

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Kibble, E.A., Sarkar-Tyson, M., Coombs, G.W., Kahler, C.M. (2019). The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp.. In: Seib, K., Peak, I. (eds) Neisseria meningitidis. Methods in Molecular Biology, vol 1969. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9202-7_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9202-7_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9201-0

  • Online ISBN: 978-1-4939-9202-7

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