Abstract
Drug screening using high-resolution mass spectrometers, including quadrupole time-of-flight mass analyzers (QqTOFs), is becoming increasingly popular due to the additional flexibility that these instruments offer laboratories. Liquid chromatography (LC) coupled to TOF, as in an LC-QqTOF, offers comparable sensitivity and a shortened method development time relative to triple quadrupole-based mass spectrometry. In addition, LC-QqTOF data that are collected in untargeted mode can be analyzed retrospectively to detect additional compounds that were not predefined targets. Much of the power of LC-QqTOF lies in data processing, and the data analysis workflow that a lab uses must be adequately validated.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Thoren KL, Colby JM, Shugarts SB, Wu AHB, Lynch KL (2016) Comparison of information-dependent acquisition on a tandem quadrupole TOF vs a triple quadrupole linear ion trap mass spectrometer for broad-spectrum drug screening. Clin Chem 62:170–178
Bade R, Rousis NI, Bijlsma L, Gracia-Lor E, Castiglioni S, Sancho JV et al (2015) Screening of pharmaceuticals and illicit drugs in wastewater and surface waters of Spain and Italy by high resolution mass spectrometry using UHPLC-QTOF MS and LC-LTQ-Orbitrap MS. Anal Bioanal Chem 407:8979
González-Mariño I, Gracia-Lor E, Bagnati R, Martins CPB, Zuccato E, Castiglioni S (2016) Screening new psychoactive substances in urban wastewater using high resolution mass spectrometry. Anal Bioanal Chem 408:4297
Moschet C, Piazzoli A, Singer H, Hollender J (2013) Alleviating the Reference standard dilemma using a systematic exact mass suspect screening approach with liquid chromatography-high resolution mass spectrometry. Anal Chem 85:10312
Colby JM, Thoren KL, Lynch KL (2016) Optimization and validation of high-resolution mass spectrometry data analysis parameters. J Anal Toxicol 1:1–5
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Colby, J.M., Lynch, K.L. (2019). Drug Screening Using Liquid Chromatography Quadrupole Time-of-Flight (LC-QqTOF) Mass Spectrometry. In: Langman, L., Snozek, C. (eds) LC-MS in Drug Analysis. Methods in Molecular Biology, vol 1872. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8823-5_17
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8823-5_17
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8822-8
Online ISBN: 978-1-4939-8823-5
eBook Packages: Springer Protocols