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The Use of Somatic Hypermutation for the Affinity Maturation of Therapeutic Antibodies

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Book cover Antibody Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1827))

Abstract

The engineering of antibodies and antibody fragments for affinity maturation, stability, and other biophysical characteristics is a common aspect of therapeutic development. Maturation of antibodies in B cells during the adaptive immune response is the result of a process called somatic hypermutation (SHM), in which the activation-induced cytidine deaminase (AID) acts to introduce mutations into immunoglobulin (Ig) genes. Iterative selection and clonal expansion of B cells containing affinity-enhancing mutations drive an increase in the overall affinity of antibodies. Here we describe the use of SHM coupled with mammalian cell surface display for the maturation of antibodies in vitro and the complementarity of these methods with the mining of immune lineages using next-generation sequencing (NGS).

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Author information

Authors and Affiliations

  1. Clinical and Translational Science Institute, UCLA David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA

    Peter M. Bowers & William J. Boyle

  2. Department of Molecular and Medical Pharmacology, California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA, USA

    Robert Damoiseaux

Authors
  1. Peter M. Bowers
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  2. William J. Boyle
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  3. Robert Damoiseaux
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Corresponding author

Correspondence to Peter M. Bowers .

Editor information

Editors and Affiliations

  1. Immunology Division, Garvan Institute of Medical Research, Darlinghurst, NSW, Australia

    Damien Nevoltris

  2. Institut Paoli-Calmettes, CRCM, Aix Marseille University, CNRS, INSERM, Marseille, France

    Patrick Chames

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Bowers, P.M., Boyle, W.J., Damoiseaux, R. (2018). The Use of Somatic Hypermutation for the Affinity Maturation of Therapeutic Antibodies. In: Nevoltris, D., Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 1827. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8648-4_24

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  • DOI: https://doi.org/10.1007/978-1-4939-8648-4_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8647-7

  • Online ISBN: 978-1-4939-8648-4

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