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Type 2 Immunity pp 135–151Cite as

Characterization of Mouse γδ T Cell Subsets in the Setting of Type-2 Immunity

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1799))

Abstract

Accumulating evidence indicates that γδ T cells are a critical component of type-2 immunity. However, the role of these cells in type-2 immune responses seems to be divergent. γδ T cells are heterogeneous lymphocytes that can be further divided into TCR-Vγ/δ definable subsets. Different subsets have distinct and sometimes opposite function during immune responses. In this chapter, we describe the detailed protocol for characterization of γδ T cell subsets in a mouse model of ovalbumin (OVA)/alum-induced type-2 immunity. Our protocol includes identifying γδ T cell subsets by flow cytometry, functionally inactivating individual subsets in vivo, purifying γδ T cell subsets, and using adoptive cell transfer to explore the role of individual subsets in OVA/alum-induced IgE responses.

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Acknowledgments

This work was supported by a NFSC grant 81670825 to Y.H., NIH grants R21AI095765 and R21AI122135 to W.K.B., and NIH grant R01 EY021199 to RLO.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China

    Wanjiang Zeng

  2. Department of Biomedical Research, National Jewish Health, Denver, CO, USA

    Rebecca L. O’Brien & Willi K. Born

  3. Department of Immunology and Microbiology, University of Colorado Health Sciences Center, Aurora, CO, USA

    Rebecca L. O’Brien & Willi K. Born

  4. Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China

    Yafei Huang

  5. Joint Laboratory for Stem Cell Engineering and Technology Transfer, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China

    Yafei Huang

Authors
  1. Wanjiang Zeng
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  2. Rebecca L. O’Brien
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  3. Willi K. Born
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  4. Yafei Huang
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Editor information

Editors and Affiliations

  1. Department of Biomedical Research, National Jewish Health, Denver, CO, USA

    R. Lee Reinhardt

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Zeng, W., O’Brien, R.L., Born, W.K., Huang, Y. (2018). Characterization of Mouse γδ T Cell Subsets in the Setting of Type-2 Immunity. In: Reinhardt, R. (eds) Type 2 Immunity. Methods in Molecular Biology, vol 1799. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7896-0_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7896-0_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7895-3

  • Online ISBN: 978-1-4939-7896-0

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