Abstract
This chapter serves as a summary of various media constituents for orchid seed germination and micropropagation. The fifteen commonly used media are arranged into individual tables listing compounds in both mg/L and molar concentration. A comparative table of mineral salt content is also included. Recent publications on the beneficial effects of organic additives such as chitosan, coconut water, banana, and potato are listed to serve as a quick reference source on the subject.
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References
Arditti J (2008) Micropropagation of orchids, 2nd edn. Blackwell Publishing, Oxford
Chugh S, Guha S, Rao IU (2009) Micropropagation of orchids: a review on the potential of different explants. Sci Hortic 122:507–520
Teixeira da Silva JA (2013) Orchids: advances in tissue culture, genetics, phytochemistry and transgenic biotechnology. Floricult Ornament Biotechnol 7:1–52
Dodds JH, Roberts LW (1995) Experiments in plant tissue culture. Cambridge University Press, Cambridge
Owen HR, Miller R (1992) An examination and correction of plant tissue culture basal medium formulations. Plant Cell Tissue Org Cult 28:147–150
de Fossard RA (1976) Tissue culture for plant propagators. Armidale, NSW, Dept. of Botany, University of New England
Rasmussen HN (1995) Terrestrial orchids: from seed to mycotrophic plant. Cambridge University Press, Cambridge
Kano K (1965) Studies on the media for orchid seed germination. Mem Fac Agric Kagawa Univ 20:1–68
Tsukamoto Y, Kano K, Katsuura T (1963) Instant media for orchid seed germination. Am Orchid Soc Bull 32:354–355
Winarto B, Teixeira da Silva JAT (2015) Use of coconut water and fertilizer for in vitro proliferation and plantlet production of Dendrobium ‘Gradita 31’. In Vitro Cell Dev Biol Plant 51:303–314
Mcgranahan GH, Driver JA, Tulecke W (1987) Tissue culture of Juglans. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry. Martinus Nijhoff Publishers, Boston, pp 261–271
Stewart SL, Kane ME (2006) Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tissue Organ Cult 86:147–158
Kauth PJ, Dutra D, Johnson TR, Stewart SL, Kane ME, Vendrame W (2008) Techniques and applications of in vitro orchid seed germination. In: Teixeira da Silva J (ed) Floriculture, ornamental and plant biotechnology: advances and topical issues. Global Science Books, Middlesex, pp 375–391
Thomale H (1954) Die Orchideen. Verlag Eugen Ulmer, Stuttgart
Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirement of suspensions cultures of soybean root cells. Exp Cell Res 50:151–158
Driver JA, Kuniyuki AH (1984) In vitro propagation of paradox walnut rootstock. Hortic Sci 19:507–509
Harvais G (1982) An improved culture medium for growing the orchid Cypripedium reginae axenically. Can J Bot 60:2547–2555
Vacin EF, Went FW (1949) Some pH changes in nutrient solutions. Bot Gaz 110:605–613
Lindemann EGP, Gunckel JE, Davidson OW (1970) Meristem culture of Cattleya. Am Orchid Soc Bull 39:1002–1004
Malmgren S (1996) Orchid propagation: theory and practice. In: Allen C (ed) Proceedings of the North American terrestrial orchids: propagation and production conference. Washington, National Arboretum, pp 63–72
Mitra GC, Prasad RN, Choudhury AR (1976) Inorganic correlated salts and differentiation of protocorms in seed of an orchid (Dendrobium fimbriatum) and correlated changes in its free amino acid context. Indian J Exp Biol 14:350–351
Tokuhara K, Mii M (1993) Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds. Plant Cell Rep 13:7–11
Schenk RV, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204
Van Waes JM, Deberg PC (1986) In vitro germination of some Western European orchids. Physiol Plant 67:253–261
Kongbangkerd A, Watthana S, Srimuang KO (2016) Influence of organic supplements on growth and development of in vitro shoots of Bulbophyllum dhaninivatii Seidenf. Appl Mech Mater 855:42–46
Huh YS, Lee JK, Nam SY, Paek KY, Suh GU (2016) Improvement of asymbiotic seed germination and seedling development of Cypripedium macranthos Sw. with organic additives. J Plant Biotechnol 43:138–145
Zhang Y, Lee Y-I, Deng L, Zhao S (2013) Asymbiotic germination of immature seeds and the seedling development of Cypripediu macranthos Sw., an endangered lady’s slipper orchid. Sci Hortic 164:130–136
Parthibhan S, Rao MV, Kumar TS (2015) In vitro regeneration from protocorms in Dendrobium aqueum Lindley—an imperiled orchid. J Genet Eng Biotechnol 13:227–233
Cui HY, Murthy HN, Moh SH, Cui YY, Paek KY (2015) Establishment of protocorm suspension cultures of Dendrobium candidum for the production of bioactive compounds. Hortic Environ Biotechnol 56:114–122
Vyas S, Guha S, Bhattacharya M, Rao IU (2009) Rapid regeneration of plants of Dendrobium lituiflorum Lindl. (Orchidaceae) by using banana extract. Sci Hortic 121:32–37
Islam MO, Islam MS, Saleh MA (2016) Effect of banana extract on growth and development of protocorm like bodies in Dendrobium sp. orchid. Agriculturists 13:101
Gnasekaran P, Rathinam X, Sinniah UR, Subramaniam S (2010) A study on the use of organic additives on the protocorm-like bodies (PLBS) growth of Phalaenopsis violacea orchid. J Phytology 2:29–33
David D, Jawan R, Marbawi H, Gansau JA (2015) Organic additives improves the in vitro growth of native orchid Vanda helvola Blume. Not Sci Biol 7:192–197
Gnasekaran P, Poobathy R, Mahmood M, Samian MR, Subramaniam S (2012) Effects of complex organic additives on improving the growth of PLBs of Vanda Kasem’s Delight. Aust J Crop Sci 6:1245–1248
Islam M, Akter M, Prodhan A (2012) Effect of potato extract on in vitro seed germination and seedling growth of local Vanda roxburgii orchid. J Bangladesh Agric Univ 9:211–215
Pacek-Bieniek A, Dyduch-Siemińska M, Rudaś M (2010) Influence of activated charcoal on seed germination and seedling development by the asymbiotic method in Zygostates grandiflora (Lindl.) Mansf. (Orchidaceae). Folia Hortic 22:45–50
JATD S, Uthairatanakij A, Obsuwan K, Shimasaki K, Tanaka M (2013) Elicitors (Chitosan and Hyaluronic Acid) affect protocorm-like body formation in hybrid Cymbidium. Floricult Ornament Biotechnol 7:77–81
Tantasawat P, Wannajindaporn A, Chantawaree C, Wangpunga C, Poomsom K, Sorntip A (2010) Chitosan stimulates growth of micropropagated Dendrobium plantlets. Acta Hortic 878:205–212
Pornpienpakdee P, Singhasurasak R, Chaiyasap P, Pichyangkura R, Bunjongrat R, Chadchawan S, Limpanavech P (2010) Improving the micropropagation efficiency of hybrid Dendrobium orchids with chitosan. Sci Hortic 124:490–499
Obsuwan K, Sawangsri K, Yoodee S, Uthairatanakij A (2010) Effects of chitosan concentration on in vitro growth of Dendrobium hybrid seedlings. Acta Hortic 878:289–294
Kananont N, Pichyangkura R, Chanprame S, Chadchawana S, Limpanavech P (2010) Chitosan specificity for the in vitro seed germination of two Dendrobium orchids (Asparagales: Orchidaceae). Sci Hortic 124:239–247
Rahmah S, Wiendi NMA, Suwarno WB, Krisantini K (2015) Proliferation of Protocorm-like bodies of Dendrobium mannii and Dendrobium mirbelianum in chitosan-containing media in vitro. J Trop Crop Sci 2:22–28
Nge KL, Nwe N, Chandrkrachang S, Stevens WF (2006) Chitosan and as a growth stimulator in orchid tissue culture. Plant Sci 170:1185–1190
Restanto DP, Santoso B, Kriswanto B, Supardjono S (2016) The application of chitosan for Protocorm like bodies (PLB) induction of orchid (Dendrobium sp.) in vitro. Agric Agric Sci Proc 9:462–468
Samarfard S, Kadir MA, Kadzimin SB, Saud HM, Ravanfar SA, Danaee M (2014) In vitro propagation and detection of somaclonal variation in Phalaenopsis gigantea as affected by chitosan and thidiazuron combinations. Hortic Sci 49:82–88
Samarfard S, Kadir MA, Kadzimin SB, Ravabfar S, Saud HM (2013) Genetic stability of in vitro multiplied Phalaenopsis gigantea protocorm-like bodies as affected by chitosan. Not Bot Horti Agrobo 41:177–183
Obsuwan K, Yoodee S, Uthairatanakij A (2010) Application of chitosan on in vitro growth of Rhynchostylis gigantea protocorms and seedlings. Acta Hortic 878:283–228
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Park, J., Yeung, E.C. (2018). Orchid Seed Germination and Micropropagation II: Media Information and Composition. In: Lee, YI., Yeung, ET. (eds) Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols. Springer Protocols Handbooks. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7771-0_6
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DOI: https://doi.org/10.1007/978-1-4939-7771-0_6
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