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Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions

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Book cover Potassium Channels

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1684))

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Abstract

The gating of potassium ion (K+) channels is regulated by various kinds of protein-protein interactions (PPIs). Structural investigations of these PPIs provide useful information not only for understanding the gating mechanisms of K+ channels, but also for developing the pharmaceutical compounds targeting K+ channels. Here, we describe a nuclear magnetic resonance spectroscopic method, termed the cross saturation (CS) method, to accurately determine the binding surfaces of protein complexes, and its application to the investigation of the interaction between a G protein-coupled inwardly rectifying K+ channel and a G protein α subunit.

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Acknowledgments

This work was supported in part by grants from the Japan New Energy and Industrial Technology Development Organization (NEDO) and the Ministry of Economy, Trade, and Industry (METI) (to I.S.), a Grant-in-Aid for Scientific Research on Priority Areas from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (MEXT) (to I.S.), the Development of core technologies for innovative drug development based upon IT from Japan Agency for Medical Research and development, AMED (to I.S.), JSPS KAKENHI Grant Number JP16H01368 (to M.O.), a grant from The Vehicle Racing Commemorative Foundation (to M.O.), and a grant from Nagase Science Technology Foundation (to M.O.).

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan

    Yuki Toyama, Yoko Mase, Hanaho Kano, Mariko Yokogawa, Masanori Osawa & Ichio Shimada

  2. Japan Biological Informatics Consortium (JBiC), Aomi, Koto-ku, Tokyo, 135-0064, Japan

    Yuki Toyama

  3. Keio University Faculty of Pharmacy, Shibakoen, Minato-ku, Tokyo, 105-8512, Japan

    Mariko Yokogawa & Masanori Osawa

Authors
  1. Yuki Toyama
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  2. Yoko Mase
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  3. Hanaho Kano
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  4. Mariko Yokogawa
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  5. Masanori Osawa
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  6. Ichio Shimada
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Corresponding author

Correspondence to Ichio Shimada .

Editor information

Editors and Affiliations

  1. Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, Oregon, USA

    Show-Ling Shyng

  2. Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, Oregon, USA

    Francis I. Valiyaveetil

  3. The Vollum Institute, Oregon Health and Science University, Portland, Oregon, USA

    Matt Whorton

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Toyama, Y., Mase, Y., Kano, H., Yokogawa, M., Osawa, M., Shimada, I. (2018). Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions. In: Shyng, SL., Valiyaveetil, F., Whorton, M. (eds) Potassium Channels. Methods in Molecular Biology, vol 1684. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7362-0_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7362-0_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7361-3

  • Online ISBN: 978-1-4939-7362-0

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