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Animal Models for Pseudomonas aeruginosa Quorum Sensing Studies

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Quorum Sensing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1673))

Abstract

Quorum sensing (QS) systems play global regulatory roles in bacterial virulence. They synchronize the expression of multiple virulence factors and they control and modulate bacterial antibiotic tolerance systems and host defense mechanisms. Therefore, it is important to obtain knowledge about QS modes of action and to test putative therapeutics that may interrupt QS actions in the context of infections. This chapter describes methods to study bacterial pathogenesis in murine acute and persistent/relapsing infection models, using the Gram-negative bacterial pathogen Pseudomonas aeruginosa as an example. These infection models can be used to probe bacterial virulence functions and in mechanistic studies, as well as for the assessment of the therapeutic potential of antibacterials, including anti-virulence agents.

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Author information

Author notes

  1. Damien Maura and Arunava Bandyopadhaya contributed equally to this work.

Authors and Affiliations

  1. Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

    Damien Maura, Arunava Bandyopadhaya & Laurence G. Rahme Ph.D.

  2. Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA

    Damien Maura, Arunava Bandyopadhaya & Laurence G. Rahme Ph.D.

  3. Shriners Hospitals for Children Boston, Boston, MA, USA

    Damien Maura, Arunava Bandyopadhaya & Laurence G. Rahme Ph.D.

Authors
  1. Damien Maura
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  2. Arunava Bandyopadhaya
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  3. Laurence G. Rahme Ph.D.
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Corresponding author

Correspondence to Laurence G. Rahme Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Science, University Roma Tre, Rome, Italy

    Livia Leoni

  2. Department of Science, University Roma Tre, Rome, Italy

    Giordano Rampioni

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Maura, D., Bandyopadhaya, A., Rahme, L.G. (2018). Animal Models for Pseudomonas aeruginosa Quorum Sensing Studies. In: Leoni, L., Rampioni, G. (eds) Quorum Sensing. Methods in Molecular Biology, vol 1673. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7309-5_18

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  • DOI: https://doi.org/10.1007/978-1-4939-7309-5_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7308-8

  • Online ISBN: 978-1-4939-7309-5

  • eBook Packages: Springer Protocols

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