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Development of Sex-Specific PCR-Based Markers in Date Palm

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Date Palm Biotechnology Protocols Volume II

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1638))

Abstract

Molecular markers are used efficiently in the development and identification of gender-specific PCR-based markers in date palm. There is mounting evidence that different marker systems vary in their mechanisms of detecting polymorphism and genome coverage. Therefore, they could complement each other to generate accurate sex-specific markers in date palm. This chapter describes the uses of PCR-based molecular markers to develop and identify the gender in different date palm genotypes; these are amplified fragment length polymorphism (AFLP), start codon targeted polymorphism (SCoT), conserved DNA-derived polymorphism (CDDP), intron-targeted amplified polymorphism (ITAP), and random amplified polymorphic DNA (RAPD). Also described is how to characterize the identified markers by Sanger sequencing and to explore their functions through alignment of their sequences with the Genbank databases.

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Acknowledgment

This work was carried out in the Molecular Genetic s and Genome Mapping Laboratory (MGGM), Agricultural Genetic Engineering Research Institute (AGERI) , ARC and funded by the Science and Technology Development Fund (STDF), Ministry of Scientific Research, Egypt. Contracts No. 406 and 4607.

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Authors and Affiliations

  1. Molecular Genetics and Genome Mapping Laboratory (MGGM), Agricultural Genetic Engineering Research Institute (AGERI), ARC, P.O. Box 12619, Giza, Egypt

    Mohamed A. M. Atia, Morad M. Mokhtar & Sami S. Adawy

  2. Plant Biotechnology Department, National Research Center, Dokki, Giza, Egypt

    Mahmoud M. Sakr

Authors
  1. Mohamed A. M. Atia
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  2. Mahmoud M. Sakr
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  3. Morad M. Mokhtar
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  4. Sami S. Adawy
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Corresponding author

Correspondence to Mohamed A. M. Atia .

Editor information

Editors and Affiliations

  1. Department of Agricultural Biotechnology, King Faisal University, Al-Hassa, Saudi Arabia

    Jameel M. Al-Khayri

  2. Agricultural Sciences, University of Helsinki, Helsinki, Finland

    S.Mohan Jain

  3. Cincinnati, Ohio, USA

    Dennis V. Johnson

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© 2017 Springer Science+Business Media LLC

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Atia, M.A.M., Sakr, M.M., Mokhtar, M.M., Adawy, S.S. (2017). Development of Sex-Specific PCR-Based Markers in Date Palm. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume II. Methods in Molecular Biology, vol 1638. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7159-6_19

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  • DOI: https://doi.org/10.1007/978-1-4939-7159-6_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7158-9

  • Online ISBN: 978-1-4939-7159-6

  • eBook Packages: Springer Protocols

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