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Bioprinting of 3D Tissue Models Using Decellularized Extracellular Matrix Bioink

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3D Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1612))

Abstract

Bioprinting provides an exciting opportunity to print and pattern all the components that make up a tissue—cells and extracellular matrix (ECM) material—in three dimensions (3D) to generate tissue analogues. A large number of materials have been used for making bioinks; however, majority of them cannot represent the complexity of natural ECM and thus are unable to reconstitute the intrinsic cellular morphologies and functions. We present here a method for making of bioink from decellularized extracellular matrices (dECMs) and a protocol for bioprinting of cell-laden constructs with this novel bioink. The dECM bioink is capable of providing an optimized microenvironment that is conducive to the growth of 3D structured tissue. We have prepared bioinks from different tissues, including adipose, cartilage and heart tissues and achieved high cell viability and functionality of the bioprinted tissue structures using our novel bioink.

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References

  1. Derby B (2012) Printing and prototyping of tissues and scaffolds. Science 338:921–926

    Article  CAS  PubMed  Google Scholar 

  2. Griffith LG, Naughton G (2002) Tissue engineering—current challenges and expanding opportunities. Science 295:1009–1014

    Article  CAS  PubMed  Google Scholar 

  3. Gaetani R, Doevendans PA, Metz CHG et al (2012) Cardiac tissue engineering using tissue printing technology and human cardiac progenitor cells. Biomaterials 33:1782–1790

    Article  CAS  PubMed  Google Scholar 

  4. Falconnet D, Csucs G, Michelle Grandin H et al (2006) Surface engineering approaches to micropattern surfaces for cell-based assays. Biomaterials 27:3044–3063

    Article  CAS  PubMed  Google Scholar 

  5. Chang R, Nam J, Sun W (2008) Direct cell writing of 3D microorgan for in vitro pharmacokinetic model. Tissue Eng Part C Methods 14:157–166

    Article  CAS  PubMed  Google Scholar 

  6. Fischbach C, Chen R, Matsumoto T et al (2007) Engineering tumors with 3D scaffolds . Nat Methods 4:855–860

    Google Scholar 

  7. Derby B (2008) Bioprinting: inkjet printing proteins and hybrid cell-containing materials and structures. J Mater Chem 18:5717–5721

    Article  CAS  Google Scholar 

  8. Kundu J, Shim J-H, Jang J et al (2013) An additive manufacturing-based PCL–alginate–chondrocyte bioprinted scaffold for cartilage tissue engineering. J Tissue Eng Regen Med 9:1286–1297

    Google Scholar 

  9. Jung JW, Kang H-W, Kang T-Y et al (2012) Projection image-generation algorithm for fabrication of a complex structure using projection-based Microstereolithography. Int J Precis Eng Manuf 13:445–449

    Google Scholar 

  10. Seol Y-J, Kang T-Y, Cho D-W (2012) Solid freeform fabrication technology applied to tissue engineering with various biomaterials. Soft Matter 8:1730–1735

    Google Scholar 

  11. Ferris C, Gilmore K, Wallace G et al (2013) Biofabrication: an overview of the approaches used for printing of living cells. Appl Microbiol Biotechnol 97:4243–4258

    Article  CAS  PubMed  Google Scholar 

  12. Billiet T, Gevaert E, De Schryver T et al (2014) The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability. Biomaterials 35:49–62

    Article  CAS  PubMed  Google Scholar 

  13. Wang X, Yan Y, Pan Y et al (2006) Generation of three-dimensional hepatocyte/gelatin structures with rapid prototyping system. Tissue Eng 12:83–90

    Article  CAS  PubMed  Google Scholar 

  14. Yan Y, Wang X, Xiong Z et al (2005) Direct construction of a three-dimensional structure with cells and hydrogel. J Bioact Compat Polym 20:259–269

    Article  CAS  Google Scholar 

  15. Pati F, Gantelius J, Svahn HA (2016) 3D bioprinting of tissue/organ models. Angew Chem Int Ed Engl 55:4650–4665

    Article  CAS  PubMed  Google Scholar 

  16. Sellaro TL, Ranade A, Faulk DM et al (2010) Maintenance of human hepatocyte function in vitro by liver-derived extracellular matrix gels. Tissue Eng Part A 16:1075–1082

    Article  CAS  PubMed  Google Scholar 

  17. Frantz C, Stewart KM, Weaver VM (2010) The extracellular matrix at a glance. J Cell Sci 15:4195–4200

    Article  Google Scholar 

  18. Pati F, Jang J, Ha D-H et al (2014) Printing three dimensional tissue analogues with decellularized extracellular matrix bioink. Nat Commun 5:3935

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Pati F, Ha D-H, Jang J et al (2015) Biomimetic 3D tissue printing for soft tissue regeneration. Biomaterials 62:164–175

    Article  CAS  PubMed  Google Scholar 

  20. Jin-Hyung S, Jong Young K, Min P et al (2011) Development of a hybrid scaffold with synthetic biomaterials and hydrogel using solid freeform fabrication technology. Biofabrication 3:034102

    Google Scholar 

  21. Park I-S, Han M, Rhie J-W et al (2009) The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism. Biomaterials 30:6835–6843

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was partially supported by the Early Career Research (ECR) grant awarded by Science and Engineering Research Board, Department of Science and Technology, Government of India (ECR/2015/000458) and National Research Foundation (NRF) of Korea grant funded by the Korean government (MSIP) (No. 2010-0018294).

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Authors and Affiliations

  1. Department of Biomedical Engineering, Indian Institute Technology Hyderabad, Kandi, Sangareddy, 502285, Telangana, India

    Falguni Pati

  2. Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam ro, Nam-gu, Pohang, Kyungbuk, 790-784, Republic of Korea

    Dong-Woo Cho

Authors
  1. Falguni Pati
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  2. Dong-Woo Cho
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Corresponding author

Correspondence to Falguni Pati .

Editor information

Editors and Affiliations

  1. Department of Histology and Embryology, Masaryk University, Brno, Czech Republic

    Zuzana Koledova

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Pati, F., Cho, DW. (2017). Bioprinting of 3D Tissue Models Using Decellularized Extracellular Matrix Bioink. In: Koledova, Z. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 1612. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7021-6_27

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  • DOI: https://doi.org/10.1007/978-1-4939-7021-6_27

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7019-3

  • Online ISBN: 978-1-4939-7021-6

  • eBook Packages: Springer Protocols

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