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Structural Stringency of Cholesterol for Membrane Protein Function Utilizing Stereoisomers as Novel Tools: A Review

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Cholesterol Homeostasis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1583))

Abstract

Cholesterol is an important lipid in the context of membrane protein function. The function of a number of membrane proteins, including G protein-coupled receptors (GPCRs) and ion channels, has been shown to be dependent on membrane cholesterol. However, the molecular mechanism underlying such regulation is still being explored. In some cases, specific interaction between cholesterol and the protein has been implicated. In other cases, the effect of cholesterol on the membrane properties has been attributed for the regulation of protein function. In this article, we have provided an overview of experimental approaches that are useful for determining the degree of structural stringency of cholesterol for membrane protein function. In the process, we have highlighted the role of immediate precursors in cholesterol biosynthetic pathway in the function of membrane proteins. Special emphasis has been given to the application of stereoisomers of cholesterol in deciphering the structural stringency required for regulation of membrane protein function. A comprehensive examination of these processes would help in understanding the molecular basis of cholesterol regulation of membrane proteins in subtle details.

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Acknowledgments

Work in A.C.’s laboratory was supported by the Council of Scientific and Industrial Research (Govt. of India) Network project BSC0115. A.C. is an Adjunct Professor of Tata Institute of Fundamental Research (Mumbai), RMIT University (Melbourne, Australia), Indian Institute of Technology (Kanpur), and Indian Institute of Science Education and Research (Mohali). A.C. gratefully acknowledges J.C. Bose Fellowship (Dept. of Science and Technology, Govt. of India). Some of the work described in this article was carried out by former members of A.C.’s research group whose contributions are gratefully acknowledged. We thank G. Aditya Kumar for help in making figures, and members of the Chattopadhyay laboratory for critically reading the manuscript.

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  1. CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500 007, India

    Md. Jafurulla & Amitabha Chattopadhyay

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  1. Md. Jafurulla
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  2. Amitabha Chattopadhyay
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Correspondence to Amitabha Chattopadhyay .

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  1. Faculty of Pharmacy, The University of Sydney, Sydney, New South Wales, Australia

    Ingrid C. Gelissen

  2. School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales, Australia

    Andrew J. Brown

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Jafurulla, M., Chattopadhyay, A. (2017). Structural Stringency of Cholesterol for Membrane Protein Function Utilizing Stereoisomers as Novel Tools: A Review. In: Gelissen, I., Brown, A. (eds) Cholesterol Homeostasis. Methods in Molecular Biology, vol 1583. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6875-6_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6875-6_3

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