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Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function

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Thermogenic Fat

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1566))

Abstract

Viral vectors have become widely used tools for genetic manipulation of adipose tissues to understand the biology and function of adipocytes in metabolism. There are a number of different viral vectors commonly used: retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). Here, we review examples from the literature and describe methods to transduce adipocytes and adipose tissues using retrovirus, lentivirus, adenovirus, and AAV to ascertain gene function in adipose biology.

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Notes

  1. 1.

    Viral particles can be added to cultured cells during the preadipocyte stage to test for effects on adipogenesis or to differentiated adipocytes to assess for effects on gene expression and adipocyte function. For retrovirus , viral particles will need to be added during the preadipocyte stage when the cells are actively undergoing replication

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Authors and Affiliations

  1. Metabolic Health Center, Division of Cardiology, Department of Medicine, Weill Cornell Medicine, 413 East 69th Street, New York, NY, 10021, USA

    Nicolás Gómez-Banoy & James C. Lo

Authors
  1. Nicolás Gómez-Banoy
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  2. James C. Lo
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Corresponding author

Correspondence to James C. Lo .

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Editors and Affiliations

  1. Department of Molecular and Integrative Physiology, Life Sciences Institute, University of Michigan, Ann Arbor, Michigan, USA

    Jun Wu

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Gómez-Banoy, N., Lo, J.C. (2017). Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function. In: Wu, J. (eds) Thermogenic Fat. Methods in Molecular Biology, vol 1566. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6820-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6820-6_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6819-0

  • Online ISBN: 978-1-4939-6820-6

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