Abstract
In this chapter, we introduce a completely intraoperative procedure for obtaining a patient-derived biomaterial in cell therapy and tissue engineering applications. An automated device for processing human peripheral-blood ensures a reproducible method for retrieving the patient’s cellular-rich as well as cellular-poor plasma. By substituting calcium for animal-derived thrombin, we engineer a completely autologous hydrogel that eliminates the risk of disease transmission and lowers FDA regulation hurdles. Through this chapter, we will discuss a bedside protocol developed to prepare a patient-derived hydrogel. This method can be effectively used to develop a completely intraoperative tissue engineering strategy (CITES) that can be easily translated into the clinic for surgical use.
*Authors contributed equally.
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References
Nukavarapu SP, Freeman JW, Laurencin CT (eds) (2015) Regenerative engineering of musculoskeletal tissues and interfaces. Elsevier, New York, NY
Amini AR, Laurencin CT, Nukavarapu SP (2012) Bone tissue engineering: recent advances and challenges. Crit Rev Biomed Eng 40:363–408
Nukavarapu SP, Liu H, Deng T, Oyen M, Tamerler C (eds) (2013) Advances in structures, properties, and applications of biological and bioinspired materials. Materials Research Society, New York, NY
Nukavarapu SP, Dorcemus DL (2013) Osteochondral tissue engineering: current strategies and challenges. Biotechnol Adv 31(5):706–721
Mikael PE, Xin X, Urso M, Jiang X, Wang L, Barnes B, Lichtler AC, Rowe DW, Nukavarapu SP (2014) A potential translational approach for bone tissue engineering through endochondral ossification. Conf Proc IEEE Eng Med Biol Soc 2014:3925–3928
Mikael PE, Amini AR, Basu J, Arellano-Jimenez MJ, Laurencin CT, Sanders MM, Barry Carter C, Nukavarapu SP (2014) Functionalized carbon nanotube reinforced scaffolds for bone regenerative engineering: fabrication, in vitro and in vivo evaluation. Biomed Mater 9(3):035001
Nukavarapu S, Almobark A, Casettari L, Luzzi A (2015) Hydrogels: cell delivery and tissue regeneration. In: Mishra M (ed) Encyclopedia of biomedical polymers and polymer biomaterials, vol 6. Taylor & Francis, New York, pp 3841–3852
Zhu J, Marchant RE (2011) Design properties of hydrogel tissue-engineering scaffolds. Expert Rev Med Devices 8(5):607–626
Kopecek J (2007) Hydrogel biomaterials: a smart future? Biomaterials 28(34):5185–5192
Liu J, Zheng H (2015) Hydrogels for engineering of perfusable vascular networks. Int J Mol Sci 16:15997–16016
Choi D, Lee W, Jinwon P, Koh W (2008) Preparation of poly(ethylene glycol) hydrogels with different network structures for the application of enzyme immobilization. Biomed Mater Eng 18(6):345–356
Hassan CM, Peppas NA (2000) Structure and applications of poly(vinyl alcohol) hydrogels produced by conventional crosslinking or by freezing/thawing methods. Adv Polym Sci 153:37
Alfonso M, Michelle D (2013) Surface and in-depth characterization of bioresorbable poly(lactic acid) membranes and bioresorbable chitosan-based hydrogels for therapeutic drug release; Thesis; http://hdl.handle.net/10477/50530.
Zhao W, Jin X, Cong Y, Liu Y, Fu J (2012) Degradable natural polymer hydrogels for articular cartilage tissue engineering. J Chem Technol Biotechnol 88(3):327–339
Xu X, Jha AK, Harrington DA, Farach-carson MC, Jia X (2012) Hyaluronic acid-based hydrogels: from a natural polysaccharide to complex networks. Soft Matter 8(12):3280–3294
Kim I, Choi JS, Lee SH, Byeon HJ, Lee ES, Shin BS, Choi HG, Lee KC, Youn YS (2015) In situ facile-forming PEG cross-linked albumin hydrogels loaded with an apoptotic TRAIL protein. J Control Release 214:30–39
Wang E, Desai MS, Lee SW (2013) Light controlled graphene-elastin composite hydrogel actuators. Nano Lett 13:2826–2830
Tawil B, Wu B (2012) Three dimensional fiber constructs in tissue engineering. In: Hollinger JO (ed) An introduction to biomaterials, 2nd edn. CRC Taylor & Francis, Boca Raton, FL, pp 249–262
Sierpinski P, Gerrett J, Ma J, Apel P, Klorig D, Smith T, Koman LA, Atala A, Dyke MV (2008) The use of keratin biomaterials derived from human hair for the promotion of rapid regeneration of peripheral nerves. Biomaterials 29(1):118–128
YK Y, KE S, LD B (2011) Encapsulation of cardiomyocytes in a fibrin hydrogel for cardiac tissue engineering. J Vis Exp 55
Li Y, Meng H, Liu Y, Lee BP (2015) Fibrin gel as an injectable biodegradable scaffold and cell carrier for tissue engineering. Scientific World Journal 2015:685–690
QuinnJV (2005), Fibrin-based adhesives and hemostatic agents in Tissue adhesives in clinical medicine, BC Decker Inc, Second Edition. 80–97
WD S (2010) Fibrin sealant: past, present, and future: a brief review. World J Surg 34(4):632–634
Breen A, O’brien T, Pandit A (2009) Fibrin as a delivery system for therapeutic drugs and biomolecules. Tissue Eng Part B Rev 15(2):201–214
Janmey PA, Winer JP, Weisel JW (2009) Fibrin gels and their clinical and bioengineering applications. J R Soc Interface 6(30):1–10
Walsh PN (2004) Platelet coagulation-protein interactions. Semin Thromb Hemost 30(4):461–471
Cheng CM, Meyer-Massetti C, Kayser SR (2009) A review of three stand-alone topical thrombins for surgical hemostasis. Clin Ther 31:32–41
Marx RE (2001) Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dent 10:225–228
Ruszymah BH (2004) Autologous human fibrin as the biomaterial for tissue engineering. Med J Malaysia 59 Suppl B:30–1
Arteriocyte Medical Systems Inc. (2014) Magellan autologous platelet separator. http://www.arteriocyte.com/magellanreg-autologous-platelet-separator.html. Accessed20 July 2015
Christensen K, Vang S, Brady C, Isler J, Allen K, Anderson J, Holt D (2006) Autologous platelet gel: an in vitro analysis of platelet-rich plasma using multiple cycles. J Extra Corpor Technol 38(3):249–253
Acknowledgments
Dr. Nukavarapu acknowledges funding from AO Foundation (S-13-122N), Musculoskeletal Transplant Foundation (MTF), and National Science Foundation (PFI AIR-131190 & EFRI-1332329). He also acknowledges support from Connecticut Institute for Clinical and Translational Science (CICATS) through a Mentorship award (M-1). Melissa thanks Young Innovative Investigator Program (YIIP), CICATS for fellowship. The authors are grateful to Deborah and Paiyz for their help with Magellan® System and confocal imaging.
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Joshi, S.U., Barbu, R.O., Carr-Reynolds, M., Barnes, B., Nukavarapu, S.P. (2017). Patient-Derived and Intraoperatively Formed Biomaterial for Tissue Engineering. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_21
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DOI: https://doi.org/10.1007/978-1-4939-6756-8_21
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