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The Use of Chromatin Precipitation Coupled to DNA Sequencing (ChIP-Seq) for the Analysis of Zta Binding to the Human and EBV Genome

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Epstein Barr Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1532))

Abstract

Determining which components of the transcription machinery associate with the viral and cellular genome, and how this changes at specific stages of the viral life cycle is paramount to understanding how the distinct transcriptional programs associated with primary infection, latency, and disease are established and how they are reprogrammed during initiation and execution of the viral lytic replication cycle. Chromatin precipitations linked to next generation DNA sequencing (ChIP-Seq) allow for the interactions of proteins with DNA to be mapped across both viral and cellular genomes. This can be applied to viral and cellular transcription factors, coactivators and corepressors, modified histones, and modulators of chromatin.

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Acknowledgment

This work was supported by MRC MR/J001 708/1.

Author information

Authors and Affiliations

  1. School of Life Sciences, University of Sussex, JMS Bldg 3C19, Brighton, BN1 9QG, UK

    Anja Godfrey & Alison J. Sinclair

  2. V ClinBio Labs Pvt Ltd., Central Research Facility, Sri Ramachandra University, Porur, Chennai, 600 016, India

    Sharada Ramasubramanyan

Authors
  1. Anja Godfrey
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  2. Sharada Ramasubramanyan
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  3. Alison J. Sinclair
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Corresponding author

Correspondence to Alison J. Sinclair .

Editor information

Editors and Affiliations

  1. University of Szeged, Szeged, Hungary

    Janos Minarovits

  2. University of Regensburg, Regensburg, Germany

    Hans Helmut Niller

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Godfrey, A., Ramasubramanyan, S., Sinclair, A.J. (2017). The Use of Chromatin Precipitation Coupled to DNA Sequencing (ChIP-Seq) for the Analysis of Zta Binding to the Human and EBV Genome. In: Minarovits, J., Niller, H. (eds) Epstein Barr Virus. Methods in Molecular Biology, vol 1532. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6655-4_14

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  • DOI: https://doi.org/10.1007/978-1-4939-6655-4_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6653-0

  • Online ISBN: 978-1-4939-6655-4

  • eBook Packages: Springer Protocols

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