Abstract
Bone tissue is intrinsically hard and thus, it is more difficult to handle, process, and examine than soft tissues. Here, we describe an experimental model of bone regeneration and several selected protocols useful for investigating mRNA and protein expression in bone. The inhibitory function of CCN3 on membranous bone formation has been confirmed by following the protocols described herein (Fig. 1).
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References
Katsuki Y, Sakamoto K, Minamizato T et al (2008) Inhibitory effect of CT domain of CCN3/NOV on proliferation and differentiation of osteogenic mesenchymal stem cells, Kusa-A1. Biochem Biophys Res Commun 368:808–814
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Minamizato T, Sakamoto K, Liu T et al (2007) CCN3/NOV inhibits BMP-2-induced osteoblast differentiation by interacting with BMP and Notch signaling pathways. Biochem Biophys Res Commun 354:567–573
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Sakamoto, K., Matsushita, Y., Minamizato, T., Katsuki, Y., Katsube, Ki., Yamaguchi, A. (2017). The Bone Regeneration Model and Primary Osteoblastic Cell Culture Used in the Analysis of Ccn3 Transgenic and Knockout Mice. In: Takigawa, M. (eds) CCN Proteins. Methods in Molecular Biology, vol 1489. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6430-7_27
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DOI: https://doi.org/10.1007/978-1-4939-6430-7_27
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6428-4
Online ISBN: 978-1-4939-6430-7
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