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Targeted Delivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells

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Non-Viral Gene Delivery Vectors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1445))

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Abstract

The application of homing peptides to direct DNA and RNA lipoplexes to target cells is a rapidly evolving area of study, which may find application in corrective gene therapy for the treatment of neoplasms and other disorders of a genetic origin. Here, a step-wise account of the assembly and characterization of hepatocellular carcinoma cell-specific DNA lipoplexes and their cytotoxicity assessment in and delivery to the human hepatocellular carcinoma cell line HepG2 is given.

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Authors and Affiliations

  1. Discipline of Biochemistry, University of KwaZulu-Natal, Westville Campus, P. Bag X54001, Durban, 4000, South Africa

    Mario Ariatti

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  1. Mario Ariatti
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Correspondence to Mario Ariatti .

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Editors and Affiliations

  1. Polytechnic University of Milan, Milan, Italy

    Gabriele Candiani

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Ariatti, M. (2016). Targeted Delivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells. In: Candiani, G. (eds) Non-Viral Gene Delivery Vectors. Methods in Molecular Biology, vol 1445. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3718-9_7

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  • DOI: https://doi.org/10.1007/978-1-4939-3718-9_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3716-5

  • Online ISBN: 978-1-4939-3718-9

  • eBook Packages: Springer Protocols

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