Abstract
This protocol describes an enzymatic approach for isolating homogeneous cultures of pericytes from retinas of bovine source. In summary, retinas are dissected, washed, digested, filtered, cultured in specific media to select for pericytes, and finally expanded for a low passage culture of about 14 million bovine retinal pericytes (BRP) within 4–6 weeks. This protocol also describes a liposomal-based technique for transfection of BRPs.
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Acknowledgements
We thank Dr. Patricia D’Amore for her valuable discussion, insights, and guidance with the development of this method. This work was supported by NIH grants EY021624 and EY021624-03 (J.A.-V.), and American Heart Association Scientist Development Grant 12SDG8960025 (J.A.-V.).
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Primo, V.A., Arboleda-Velasquez, J.F. (2016). Isolation and Transfection of Primary Culture Bovine Retinal Pericytes. In: Martin, S., Hewett, P. (eds) Angiogenesis Protocols. Methods in Molecular Biology, vol 1430. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3628-1_7
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DOI: https://doi.org/10.1007/978-1-4939-3628-1_7
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