Abstract
Screening of viral inhibitors through induction of cytopathic effects (CPE) by conventional method has been applied for various viruses including Chikungunya virus (CHIKV), a significant arbovirus. However, it does not provide the information about cytopathic effect from the beginning and throughout the course of virus replication. Conventionally, most of the approaches are constructed on laborious end-point assays which are not capable for detecting minute and rapid changes in cellular morphology. Therefore, we developed a label-free and dynamical method for monitoring the cellular features that comprises cell attachment, proliferation, and viral cytopathogenicity, known as the xCELLigence real-time cell analysis (RTCA). In this chapter, we provide a RTCA protocol for quantitative analysis of CHIKV replication using an infected Vero cell line treated with ribavirin as an in vitro model.
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Acknowledgment
The author would like to thank University of Malaya for the UMRG flagship grant (Flagship: FL001-13HTM), High Impact Research (HIR) grant E000013-20001 and postgraduate (PPP) grant (Grant No. PG037-2013B) for funding the expenses of this work.
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Zandi, K. (2016). A Real-Time Cell Analyzing Assay for Identification of Novel Antiviral Compounds against Chikungunya Virus. In: Chu, J., Ang, S. (eds) Chikungunya Virus. Methods in Molecular Biology, vol 1426. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3618-2_23
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DOI: https://doi.org/10.1007/978-1-4939-3618-2_23
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3616-8
Online ISBN: 978-1-4939-3618-2
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