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Immunohistochemical Detection of Chikungunya Virus Antigens in Formalin-Fixed and Paraffin-Embedded Tissues

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Chikungunya Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1426))

Abstract

Immunohistochemistry is a histological technique that allows detection of one or more proteins of interest within a cell using specific antibody binding, followed by microscopic visualization of a chromogenic substrate catalyzed by peroxidase and/or alkaline phosphatase. Here, we describe a method to localize Chikungunya virus (CHIKV) antigens in formalin-fixed and paraffin-embedded infected mouse brain.

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References

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Acknowledgments

We thank Andres Merits of Tartu University, Estonia for contributing rabbit anti-CHIKV capsid antibody. The authors’ work was supported by European Union’s Seventh Framework Program (Integrated Chikungunya Research (ICRES) grant agreement no. 261202), University of Malaya (HIR grant UM.C/625/1/HIR/016, PG030-2012B), and the Ministry of Higher Education, Malaysia (FRGS grant FP032-2010A).

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Authors and Affiliations

  1. Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Chun Wei Chiam, I-Ching Sam & Yoke Fun Chan

  2. Department of Pathology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Kum Thong Wong

  3. Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Kien Chai Ong

Authors
  1. Chun Wei Chiam
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  2. I-Ching Sam
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  3. Yoke Fun Chan
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  4. Kum Thong Wong
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  5. Kien Chai Ong
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Corresponding author

Correspondence to Kien Chai Ong .

Editor information

Editors and Affiliations

  1. Dept of Microbiology and Immunology, National University of Singapore, Singapore, Singapore

    Justin Jang Hann Chu

  2. Dept of Microbiology and Immunology, National University of Singapore, Singapore, Singapore

    Swee Kim Ang

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© 2016 Springer Science+Business Media New York

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Chiam, C.W., Sam, IC., Chan, Y.F., Wong, K.T., Ong, K.C. (2016). Immunohistochemical Detection of Chikungunya Virus Antigens in Formalin-Fixed and Paraffin-Embedded Tissues. In: Chu, J., Ang, S. (eds) Chikungunya Virus. Methods in Molecular Biology, vol 1426. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3618-2_21

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  • DOI: https://doi.org/10.1007/978-1-4939-3618-2_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3616-8

  • Online ISBN: 978-1-4939-3618-2

  • eBook Packages: Springer Protocols

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