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Virus Isolation and Preparation of Sucrose-Banded Chikungunya Virus Samples for Transmission Electron Microscopy

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Chikungunya Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1426))

Abstract

Virus isolation and purification is an invaluable technique in virology to detect and characterize viruses. This chapter describes a large-scale Chikungunya virus (CHIKV) propagation and purification methods by using discontinuous sucrose gradient, and sample preparation for transmission electron microscopy. Sucrose-banding yields large quantities of high-titer (1010 pfu/ml) CHIKV stocks. Such stocks are stable for years when stored at −70 °C.

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References

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Acknowledgments

The author would like to express his deep sense of gratitude to Dr. Tomohiko Takasaki for countless discussions, and for critical review of this manuscript. I also want to thank Keiko Tanaka for her great help with TEM. I also would like to thank Dr. Polly Roy, in whose laboratory I learned the fundamentals of viral purification and TEM operation.

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Authors and Affiliations

  1. Laboratory of Neurovirology, Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuiku-ku, Tokyo, 162-8640, Japan

    Chang-Kweng Lim

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  1. Chang-Kweng Lim
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Correspondence to Chang-Kweng Lim .

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Editors and Affiliations

  1. Dept of Microbiology and Immunology, National University of Singapore, Singapore, Singapore

    Justin Jang Hann Chu

  2. Dept of Microbiology and Immunology, National University of Singapore, Singapore, Singapore

    Swee Kim Ang

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Lim, CK. (2016). Virus Isolation and Preparation of Sucrose-Banded Chikungunya Virus Samples for Transmission Electron Microscopy. In: Chu, J., Ang, S. (eds) Chikungunya Virus. Methods in Molecular Biology, vol 1426. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3618-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3618-2_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3616-8

  • Online ISBN: 978-1-4939-3618-2

  • eBook Packages: Springer Protocols

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