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Analysis of Dendritic Cell Function Using Clec9A-DTR Transgenic Mice

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Dendritic Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1423))

Abstract

The Clec9A-diphtheria toxin receptor (DTR) transgenic mouse strain provides a robust animal model to study the function of lymphoid organ-resident CD8+ dendritic cells (DCs) and nonlymphoid organ-specific CD103+ DCs in infectioous diseases and inflammation. Here we describe some basic protocols for CD8+/CD103+ DC isolation, for their in vivo depletion, and for their characterization by multi-color flow cytometry analysis. As an example for in vivo functional characterization of this DC subset, we present here the experimental cerebral malaria model. Furthermore, we illustrate advantages and pitfalls of the Clec9A-DTR system.

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Acknowledgments

We thank Klaus Karjalainen for his critical reading of the manuscript. This work was supported by National Medical Research Council grants NMMR/1253/2010, NMRC/1307/2011, and MOE2014-T2-1-011 to C.R. The authors have no conflicting financial interests.

Author information

Authors and Affiliations

  1. School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore

    Piotr Tetlak & Christiane Ruedl

Authors
  1. Piotr Tetlak
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  2. Christiane Ruedl
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Corresponding author

Correspondence to Christiane Ruedl .

Editor information

Editors and Affiliations

  1. Pavillon Pasteur, Institut Curie INSERM U932, Paris Cedex 05, France

    Elodie Segura

  2. Department of Biodefense, Tokyo Medical and Dental University, Tokyo, Japan

    Nobuyuki Onai

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Tetlak, P., Ruedl, C. (2016). Analysis of Dendritic Cell Function Using Clec9A-DTR Transgenic Mice. In: Segura, E., Onai, N. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 1423. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3606-9_20

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  • DOI: https://doi.org/10.1007/978-1-4939-3606-9_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3604-5

  • Online ISBN: 978-1-4939-3606-9

  • eBook Packages: Springer Protocols

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