Abstract
The leaf apoplast comprises the extracellular continuum outside cell membranes. A broad range of processes take place in the apoplast, including intercellular signaling, metabolite transport, and plant-microbe interactions. To study these processes, it is essential to analyze the metabolite content in apoplastic fluids. Due to the fragile nature of leaf tissues, it is a challenge to obtain apoplastic fluids from leaves. Here, methods to collect apoplastic washing fluid and guttation fluid from Arabidopsis thaliana leaves are described.
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Madsen, S.R., Nour-Eldin, H.H., Halkier, B.A. (2016). Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves. In: Fett-Neto, A. (eds) Biotechnology of Plant Secondary Metabolism. Methods in Molecular Biology, vol 1405. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3393-8_4
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DOI: https://doi.org/10.1007/978-1-4939-3393-8_4
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