Abstract
This protocol describes the use of a 16plex PCR for the purpose assessing DNA quality after isothermal whole genome amplification (WGA). In short, DNA products, generated by amplification multiple displacement amplification, are forwarded to PCR targeting 15 short tandem repeats (STR) as well as amelogenin generating up to 32 different PCR products. After amplification, the PCR products are separated via capillary electrophoresis and analyzed based on the obtained DNA profiles. Isothermal WGA products of good DNA quality will result in DNA profiles with efficiencies of >90 % of the full DNA profile.
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Acknowledgement
This work was supported by the EU SAFE Network of Excellence (LSHB-CT-2004-503243, EU 6th Framework Package) and the County of Styria, Austria.
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Kroneis, T., El-Heliebi, A. (2015). Quality Control of Isothermal Amplified DNA Based on Short Tandem Repeat Analysis. In: Kroneis, T. (eds) Whole Genome Amplification. Methods in Molecular Biology, vol 1347. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2990-0_10
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DOI: https://doi.org/10.1007/978-1-4939-2990-0_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2989-4
Online ISBN: 978-1-4939-2990-0
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